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山羊 anti-人 IgA (Heavy Chain) Antibody (Alkaline Phosphatase (AP)) - Preadsorbed

ELISA, IHC, WB Polyclonal IgG Alkaline Phosphatase (AP) F(ab')2 fragment
产品编号 ABIN5608093
发货至: 中国
  • 抗原 See all IgA products
    IgA
    片段
    F(ab')2 fragment
    抗原表位
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    Heavy Chain
    适用
    • 180
    • 62
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    宿主
    • 200
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    • 14
    • 6
    • 2
    山羊
    克隆类型
    • 302
    • 63
    多克隆
    标记
    • 91
    • 55
    • 49
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    Alkaline Phosphatase (AP)
    应用范围
    • 182
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    ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
    产品特性
    Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
    纯化方法
    Preadsorption: Solid phase absorption
    免疫原

    Immunogen: Human IgA alpha heavy chain

    亚型
    IgG
  • 应用备注

    Immunohistochemistry Dilution: 1:200-1:1,000

    Application Note: Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. This product has been assayed against 1.0 μg of Human IgA in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.

    ELISA Dilution: 1:2,000-1:10,000

    Western Blot Dilution: 1:500-1:2,500

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式

    Reconstitution Volume: 500 μL

    浓度
    0.6 mg/mL
    缓冲液

    Buffer: 0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0

    Stabilizer: 15 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.05 % (w/v) Sodium Azide

    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C
    储存方法
    Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted powder. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
    有效期
    12 months
  • 抗原
    IgA
    Abstract
    IgA 产品
    物质类
    Antibody
    背景

    Synonyms: Goat F(ab')2 anti-Human IgA (alpha chain) Antibody alkaline phosphatase Conjugation, Goat F(ab')2 anti-Human IgA alpha alk phos Conjugated Antibody

    Background: F(ab')2 Anti-Human IgA Alkaline Phosphatase Antibody generated in goat detects immunoglobulin A (alpha chain) from human. Immunoglobulin A (IgA) is an antibody that plays a critical role in mucosal immunity. IgA has two subclasses (IgA1 and IgA2) and can exist in a dimeric form called secretory IgA (sIgA). Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.

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