山羊 anti-雪貂 IgA Antibody (Alkaline Phosphatase (AP))
Rockland 618-105-006
ELISA, IHC, WB
Polyclonal
IgG
Alkaline Phosphatase (AP)
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- 抗原 See all IgA products
- IgA
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适用
- 雪貂
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- Alkaline Phosphatase (AP)
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应用范围
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- Supplier Product No.
- 618-105-006
- Supplier
- Rockland
- 原理
- Ferret IgA (alpha chain) Antibody Alkaline Phosphatase Conjugated
- 交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Ferret IgA and Ferret Serum. Specificity was confirmed by ELISA at less than 1 % cross reactivity against other Ferret heavy or light chain isotypes.
- 产品特性
- Anti-Ferret IgG IgA IgM Peroxidase Antibody generated in goat detects immunoglobulin G, A, and M from ferret. Immunoglobulin G binds to antigens and can neutralize or opsonize targets, and are predominantly involved in the secondary immune response. Immunoglobulin A (IgA) is an antibody that plays a critical role in mucosal immunity. IgA has two subclasses (IgA1 and IgA2) and can exist in a dimeric form called secretory IgA (sIgA). Immunoglobulin M, or IgM, is a pentamer composed of 5 immunoglobulin molecules linked through their F(c) domain by the J chain.
- 纯化方法
- This product was prepared from monospecific antiserum by immunoaffinity chromatography using Ferret IgA coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
- 过滤
- Sterile filtered
- 免疫原
- Optional[Immunogen]: Ferret IgA alpha heavy chain
- 亚型
- IgG
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- 应用备注
- Application Note: Anti-Ferret IgA Alk Phos conjugate is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot consistency. Immunohistochemistry Dilution: 1:200 - 1:1,000 Western Blot Dilution: 1:500 - 1:3,000 ELISA Dilution: 1:2,000 - 1:10,000 Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
, Preservative:0.1 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
- 有效期
- 12 months
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- 抗原
- IgA
- Abstract
- IgA 产品
- 物质类
- Antibody
- 背景
- Anti-Ferret IgA Alkaline Phosphatase Antibody generated in goat detects immunoglobulin A from ferret. Immunoglobulin A (IgA) is an antibody that plays a critical role in mucosal immunity. IgA has two subclasses (IgA1 and IgA2) and can exist in a dimeric form called secretory IgA (sIgA).
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