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山羊 anti-人 IgA Antibody

ELISA, IHC, WB Polyclonal IgG unconjugated F(ab')2 fragment
产品编号 ABIN5608046
发货至: 中国
  • 抗原 See all IgA products
    IgA
    片段
    F(ab')2 fragment
    适用
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    山羊
    克隆类型
    • 300
    • 67
    多克隆
    应用范围
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    ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
    原理
    F(ab')2 Human IgA (alpha chain) Antibody
    交叉反应 (详细)
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Human IgA and Human Serum.  No reaction was observed against anti-Pepsin or anti-Goat IgG F(c).  Specificity was confirmed by ELISA at less than 1 % cross reactivity against other human heavy or light chain isotypes.
    产品特性
    F(ab')2 Anti-Human IgG IgA IgM (H&L) Texas Red Antibody generated in goat detects human (heavy and light chain) immunoglobulin G, A, and M. Immunoglobulin G binds to antigens and can neutralize or opsonize targets, and are predominantly involved in the secondary immune response. Immunoglobulin A (IgA) is an antibody that plays a critical role in mucosal immunity. IgA has two subclasses (IgA1 and IgA2) and can exist in a dimeric form called secretory IgA (sIgA). Immunoglobulin M, or IgM, is a pentamer composed of 5 immunoglobulin molecules linked through their F(c) domain by the J chain. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.
    纯化方法
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.  
    过滤
    Sterile filtered
    免疫原
    Optional[Immunogen]: Human IgA alpha heavy chain
    亚型
    IgG
  • 应用备注
    Application Note: Suitable for highly specific immunological methods requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:2,000 - 1:10,000 ELISA Dilution: 1:20,000 - 1:100,000 Other: User Optimized
    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    1.3 mg/mL
    缓冲液
    Optional[Buffer]: None, Preservative:None
    储存液
    Without preservative
    储存条件
    4 °C,-20 °C
    储存方法
    Store Secondary Antibody at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.
    有效期
    12 months
  • 抗原
    IgA
    Abstract
    IgA 产品
    物质类
    Antibody
    背景
    F(ab')2 Anti-Human IgA Antibody generated in goat detects immunoglobulin A (alpha chain) from human. Immunoglobulin A (IgA) is an antibody that plays a critical role in mucosal immunity. IgA has two subclasses (IgA1 and IgA2) and can exist in a dimeric form called secretory IgA (sIgA). Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.
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