Recombinant 兔 anti-人 IgG4 Antibody
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- 抗原 See all IgG4 products
- IgG4
- 抗体类型
- Recombinant Antibody
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适用
- 人
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宿主
- 兔
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克隆类型
- 单克隆
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC)
- 产品特性
- This recombinant Human IgG4 antibody reacts to the heavy chain of human IgG4. No cross reactivity with human IgG1, IgG2, IgG3, IgM, IgA, IgD, IgE, mouse IgG, rat IgG, or goat IgG.
- 纯化方法
- Protein A purified from animal origin-free supernatant
- 免疫原
- Peptide corresponding to the hinge region of human IgG4 was used as the immunogen for this recombinant Human IgG4 antibody.
- 克隆位点
- RM120
- 亚型
- IgG
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- 应用备注
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The stated application concentrations are suggested starting points. Titration of the recombinant Human IgG4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
1. A pH 6 Citrate buffer or pH 9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.\. ELISA: 50 ng/well - 200 ng/well (Capture), 0.05-0.2 μg/mL (Detection),Western Blot: 0.5-2 μg/mL,Immunocytochemistry: 1-10 μg/mL,Immunohistochemistry: 1-10 μg/mL (1)
- 限制
- 仅限研究用
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- 缓冲液
- 1 mg/mL in PBS with 50 % glycerol, 1 % BSA and 0.09 % sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Store the recombinant Human IgG4 antibody at -20°C (with glycerol) or aliquot and store at -20°C (without glycerol).
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