山羊 anti-大鼠 IgG2a Antibody - Preadsorbed
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- 抗原 See all IgG2a products
- IgG2a
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适用
- 大鼠
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宿主
- 山羊
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克隆类型
- 多克隆
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应用范围
- ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC)
- 特异性
- The reactivity of the antiserum is directed to the subclass IgG2a as tested against rat sera and a panel of purified homogenous immunoglobulins.
- 纯化方法
- Preadsorption: Immunoaffinity adsorbed using insolubilized antigens.
- 免疫原
- Pools of purified homogenous IgG2a isolated from pooled rat serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
- 亚型
- IgG
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Mouse anti-Rat IgG2a antibody
ELISA, FACS Monoclonal 2A8F4 IgG1 unconjugated
Mouse anti-Rat IgG2a antibody (Biotin)ELISA, FACS Monoclonal 2A8F4 IgG1 Biotin
Mouse anti-Rat IgG2a antibody (PE)ELISA, FACS Monoclonal 2A8F4 IgG1 PE
Mouse anti-Rat IgG2a antibody (FITC)ELISA, FACS Monoclonal 2A8F4 IgG1 FITC
Mouse anti-Rat IgG2a antibody (Alkaline Phosphatase (AP))ELISA, IHC Monoclonal 2A 8F4 IgG1 Alkaline Phosphatase (AP)
Mouse anti-Rat IgG2a antibody (HRP)ELISA, IHC Monoclonal 2A 8F4 IgG1 HRP
Mouse anti-Rat IgG2a antibody (Cy5)ELISA, FACS Monoclonal 2A8F4 IgG1 Cy5
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- 应用备注
- As unlabelled primary or secondary reagent for indirect detection of IgG2a at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, to prepare conjugates of the user's own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established by titration before being used. Typical working dilutions in histochemistry are usually between 1:100 and 1:500, in ELISA and comparable non-precipitating antibody-binding assays between 1:500 and 1:5,000.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -24 °C.
- 浓度
- 10 mg/mL
- 缓冲液
- Purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2).
- 储存液
- Without preservative
- 注意事项
- Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7. 2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4 °C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- The lyophilized IgG (7S) fraction is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.
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- 抗原
- IgG2a
- Abstract
- IgG2a 产品
- 物质类
- Antibody
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