兔 anti-大鼠 IgG (Heavy & Light Chain) Antibody
ICC, IEP
Polyclonal
IgG
unconjugated
-
- 抗原 See all IgG products
- IgG
-
抗原表位
- Heavy & Light Chain
-
适用
- 大鼠
-
宿主
- 兔
-
克隆类型
- 多克隆
-
应用范围
- Immunocytochemistry (ICC), Immunoelectrophoresis (IEP)
- 特异性
- The reactivity of the antiserum is directed to the Fc and Fab subunits of the IgG molecule. It includes a certain degree of reactivity with other immunoglobulins via the common Fab portion.
- 产品特性
- Total protein and IgG concentration in the antiserum are comparable to those of pooled rabbit serum. Antibody titre: Precipitin titre not less than 1:128 when tested against normal rat serum in agar block titration.
- 纯化方法
-
Delipidated, heat inactivated lyophilized stable whole serum
No adsorption required. - 免疫原
- Purified normal IgG, including all known subclasses, isolated from pooled rat serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
- 亚型
- IgG
-
-
- 应用备注
- In precipitating techniques as immunoelectrophoresis, single and double radial immuno-diffusion (Mancini, Ouchterlony) to identify the presence of IgG and to determine its concen-tration. This antiserum is not intended for use in non-precipitating antibody-binding or other highly sensitive assays. For such use labelled and unlabelled cytochemical and immunoassay grade reagents meeting the necessary additional specificity and performance requirements are available.
- 限制
- 仅限研究用
-
- 状态
- Lyophilized
- 溶解方式
- It is reconstituted by adding 1 mL sterile distilled water.
- 缓冲液
- No foreign proteins added.
- 储存液
- Without preservative
- 注意事项
- Working dilutions are prepared by adding sterile phosphate buffered saline (PBS, pH 7. 2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4 °C, not refrozen, and preferably u sed the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- The lyophilized antiserum is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.
-
- 抗原
- IgG
- Abstract
- IgG 产品
- 物质类
- Antibody
-
You are here:
