山羊 anti-大鼠 IgG2b Antibody (Biotin) - Preadsorbed
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- 抗原 See all IgG2b products
- IgG2b
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适用
- 大鼠
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- Biotin
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应用范围
- ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC)
- 特异性
- Specificity of this immunoconjugate has not been tested in detail, however in double radial immunodiffusion a reaction with mouse and human has been observed.
- 纯化方法
- Preadsorption: Immunoaffinity adsorbed using insolubilized antigens.
- 免疫原
- Pools of purified homogenous IgG2b isolated from rat serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
- 亚型
- IgG
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Mouse anti-Rat IgG2b antibody
ELISA, FACS Monoclonal 2B10A8 IgG2b unconjugated
Mouse anti-Rat IgG2b antibody (PE)ELISA, FACS Monoclonal 2B10A8 IgG2b PE
Mouse anti-Rat IgG2b antibody (FITC)ELISA, FACS Monoclonal 2B10A8 IgG2b FITC
Mouse anti-Rat IgG2b antibody (Alkaline Phosphatase (AP))ELISA, IHC Monoclonal 2B 10A8 IgG2b Alkaline Phosphatase (AP)
Mouse anti-Rat IgG2b antibody (HRP)ELISA, IHC Monoclonal 2B 10A8 IgG2b HRP
Mouse anti-Rat IgG2b antibody (Cy5)ELISA, FACS Monoclonal 2B10A8 IgG2b Cy5
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- 应用备注
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In immunocytochemical and immunohistochemical staining for the detection of IgG2b at the cellular and subcellular level of appropriately treated cell and tissue substrates, to demonstrate circulating IgG2b antibodies in serodiagnostic microbiology and autoimmune diseases, to identify a specific antigen using a reference antibody of rat origin known to be of the IgG2b isotype in the middle layer of the indirect test procedure, in non-isotopic assay methodology (e.g. ELISA) to measure IgG2b in rat serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user's choice has to be used This immunoconjugate is not pre-diluted.
The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions for histochemical and cytochemical use are usually between 1:100 and 1:250, in ELISA and comparable non-precipitating antibody-binding assays between 1:1,000 and 1:10,000 depending on the method used. - 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -24 °C.
- 浓度
- 10 mg/mL
- 缓冲液
- Biotin-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2).
- 储存液
- Without preservative
- 注意事项
- Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7. 2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4 °C , not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- The lyophilized conjugate is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.
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- 抗原
- IgG2b
- Abstract
- IgG2b 产品
- 物质类
- Antibody
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