山羊 anti-大鼠 IgG (Heavy & Light Chain) Antibody
ICC, ID, IEP, RID
Polyclonal
IgG
unconjugated
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- 抗原 See all IgG products
- IgG
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抗原表位
- Heavy & Light Chain
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适用
- 大鼠
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宿主
- 山羊
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克隆类型
- 多克隆
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应用范围
- Immunocytochemistry (ICC), Immunodiffusion (ID), Immunoelectrophoresis (IEP), Radial Immunodiffusion (RID)
- 特异性
- The reactivity of the antiserum is directed to the Fc and Fab subunits of the IgG molecule. It includes a certain degree of reactivity with other immunoglobulins via the common Fab portion.
- 产品特性
- Total protein and IgG concentration in the antiserum are comparable to those of pooled goat serum. Antibody titre: Precipitin titre not less than 1:64 when tested against normal rat serum in agar block titration.
- 纯化方法
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Delipidated, heat inactivated lyophilized stable whole serum
No adsorption required. - 免疫原
- Purified normal IgG, including all known subclasses, isolated from pooled rat serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
- 亚型
- IgG
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- 应用备注
- In immunoelectrophoresis and double radial immunodiffusion to identify the presence of IgG, as secondary antibody to precipitate the immunoglobulin in normal rat serum, to prepare an immunoadsorbent for the production of Ig-free rat serum, plasma or non-Ig preparations. This antiserum is not intended for use in non-precipitating antibody-binding or other highly sensitive assays. For such uses labelled and unlabelled cytochemical and immunoassay reagents meeting the necessary additional specificity and performance requirements are available.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- It is reconstituted by adding 1 mL sterile distilled water.
- 缓冲液
- No foreign proteins added.
- 储存液
- Without preservative
- 注意事项
- Working dilutions are prepared by adding sterile phosphate buffered saline (PBS, pH 7. 2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4 °C, not refrozen, and preferably used the same da y. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- The lyophilized antiserum is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.
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- 抗原
- IgG
- Abstract
- IgG 产品
- 物质类
- Antibody
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