兔 anti-猴 IgG (Fab Region) Antibody - Preadsorbed
ELISA, IHC, ICC
Polyclonal
IgG
unconjugated
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- 抗原 See all IgG products
- IgG
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抗原表位
- Fab Region
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适用
- 猴
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宿主
- 兔
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克隆类型
- 多克隆
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应用范围
- ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC)
- 特异性
- The antiserum is reacting with the Fab subunit of intact IgG, IgA and IgM and other Ig classes of both light chain types, with their Fab or F(ab')2 subunits and with free light chains of kappa and lambda type as tested in immunoelectrophoresis and double radial immunodiffusion
- 产品特性
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Purified IgG fraction of polyclonal rabbit antiserum to monkey Fab of IgG
Antibody titre: Precipitin titre not less than 1:64 when tested against normal monkey serum in agar block titration. - 纯化方法
- Preadsorption: Immunoaffinity adsorbed using insolubilized antigens.
- 免疫原
- Purified Fab from normal IgG isolated from pooled Rhesus monkey serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
- 亚型
- IgG
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Goat anti-Monkey IgG (Chain gamma) antibody (HRP)
ELISA, IHC, WB, DB, IEM Polyclonal IgG HRP
Goat anti-Monkey IgG (Heavy Chain) antibodyELISA, IHC, WB Polyclonal IgG unconjugated
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- 应用备注
- For indirect staining of fixed cell and tissue substrates, to demonstrate the intracellular presence of free or Ig-bound subunits of both kappa and lambda type. In general this kind of products is not recommended as direct or indirect screening reagents for immunoglobulin isotypes on the surface of membranes of vital lymphoid cells. The presence of activity to the common Fab subunit may result in the staining of Ig bound to Fc-receptors on non-lymphoid cells. Combinations of isotype-specific reagents should be used instead for this purpose. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration. Typical working dilutions in histochemistry are usually between 1:50 and 1:500, in ELISA and comparable non-precipitating antibody-binding assays between 1:1000 and 1:5,000.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -24 °C.
- 浓度
- 10 mg/mL
- 缓冲液
- Purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2).
- 储存液
- Without preservative
- 注意事项
- Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7. 2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4 °C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- The lyophilized IgG fraction is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.
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- 抗原
- IgG
- Abstract
- IgG 产品
- 物质类
- Antibody
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