兔 anti-人 IgG3 Antibody
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- 抗原 See all IgG3 products
- IgG3
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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应用范围
- Immunocytochemistry (ICC), Immunodiffusion (ID), Immunoelectrophoresis (IEP)
- 特异性
- The reactivity of the antiserum is restricted to subclass specific determinants on IgG3. In immuno-electrophoresis and radial immunodiffusion, using various antiserum concentrations against normal human serum a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified IgG3. No precipitation reaction is obtained with purified other subclasses of IgG, IgA, IgM, and IgG/Fab fragmentsPrecipitating polyclonal rabbit antiserum to human IgG3, subclass specific
- 产品特性
- Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal rabbit serum. Antibody titre: Precipitin titre 1:32 when tested in agar-block immunodiffusion titration against human serum with appr
- 纯化方法
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Delipidated, heat inactivated, lyophilized, stable whole antiserum
Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies cross-reacting with other subclasses of IgG, other components of the immunoglobulin system or reacting with other serum proteins. Special attention is given to the removal of antibodies to common Ig/Fab. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum. - 免疫原
- Highly purified homogenous IgG3 isolated from human serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
- 亚型
- IgG
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Mouse anti-Human IgG3 (Hinge Region) antibody
ELISA, WB, IHC, ICC Monoclonal HP6050 IgG1 unconjugated
Mouse anti-Human IgG3 (Hinge Region) antibody (PE)ELISA, IHC, ICC Monoclonal HP6050 IgG1 PE
Mouse anti-Human IgG3 (Hinge Region) antibody (Biotin)ELISA, WB, IHC, ICC Monoclonal HP6050 IgG1 Biotin
Mouse anti-Human IgG3 (Hinge Region) antibody (FITC)ELISA, IHC, ICC Monoclonal HP6050 IgG1 FITC
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- 应用备注
- In precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgG3 in human serum or other body fluids or to determine its concentration. The antiserum is not intended for use in non-precipitating antibody-binding or other highly sensitive techniques. For such uses labelled and unlabelled cytochemical grade mouse monoclonal reagents meeting the necessary additional specificity and performance requirements are available.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Reconstitute the lyophilized antiserum by adding 1 mL sterile distilled water.
- 缓冲液
- No foreign proteins added.
- 储存液
- Without preservative
- 注意事项
- Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7. 2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4 °C, not ref rozen, and preferably used the same day.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- The lyophilized antiserum is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.
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- 抗原
- IgG3
- Abstract
- IgG3 产品
- 物质类
- Antibody
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