山羊 anti-人 IgA (Dimer), (Joining Chain) Antibody (FITC) - Preadsorbed
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- 抗原 See all IgA products
- IgA
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抗原表位
- Dimer, Joining Chain
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适用
- 人
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- FITC
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应用范围
- Immunohistochemistry (IHC), Immunocytochemistry (ICC)
- 特异性
- Tested in immunoelectrophoresis and radial immunodiffusion (Ouchterlony), this immunoconjugate shows a single precipitation reaction with totally reduced and alkylated polyclonal and monoclonal polymeric IgA, secretory IgA and IgM. A reaction of complete identity is obtained with precipitated highly purified J chain and with the single of precipitation obtained with normal human serum after two hours incubation with 9 M urea and 0.2 M mercaptoethanol at pH 8.6. In a competitive radioimmunoassay no inhibition is obtained with monomeric IgA, polyclonal IgG, reduced and alkylated alpha, mu, gamma, kappa and lambda light chains (less than 1 percent over background)
- 交叉反应 （详细）
- The antiserum does not cross-react with any other component of human plasma. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. J chains of many related specie (e.g. mouse, rabbit, dog cat sheep and swine) are known to posses a striking structural homology. of this antiserum has not been tested in detail.
- 产品特性
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Fluorescein isothiocyanate-conjugated IgG fraction of polyclonal goat antiserum to human J chain of dimeric IgA
Fluorochrome/IgG protein molar ratio (F/P) approximately 1.7. No foreign proteins added. - 纯化方法
- Preadsorption: Immunoaffinity adsorbed using insolubilized antigens
- 免疫原
- Human J chain is a polypeptide folded within the structure of the polymeric immunoglobulin, resulting in a very limited exposure of J chain antigenic determinants. The antiserum is raised against the isolated and purified J chain. J chains isolated from polymeric IgA and IgM are identical by criteria of composition, peptide maps and antigenicity, Human J chain is distinct from all other chain components of polymeric IgA and IgM. It has a unique primary structure as shown by sequence analyses. Freund's complete adjuvant is used in the first step of the immunization procedure.
- 亚型
- IgG
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Rabbit anti-Human IgA antibody
IHC Monoclonal MSVA-700R IgG Recombinant Antibody unconjugated
Rabbit anti-Human IgA (Heavy Chain) antibody - PreadsorbedELISA, IHC, WB Polyclonal IgG unconjugated
Rabbit anti-Human IgA (Heavy Chain) antibody (Biotin) - PreadsorbedELISA, IHC, WB Polyclonal IgG Biotin
Goat anti-Human IgA (Heavy Chain) antibody (Alkaline Phosphatase (AP)) - PreadsorbedELISA, IHC, WB Polyclonal IgG Alkaline Phosphatase (AP)
Rabbit anti-Human IgA (Chain alpha), (Heavy Chain) antibodyELISA, IHC, WB unconjugated
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- 应用备注
- In immunocytochemical and immunohistochemical techniques for the detection of human J chain at the cellular and subcellular level in appropriately treated cell and tissue substrates. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:10 and 1:50.
- 说明
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Excitation/Emission wavelength: 494 nm/514 nm
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 浓度
- 10 mg/mL
- 缓冲液
- FITC-coupled purified hyperimmune IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2)
- 储存液
- Without preservative
- 注意事项
- Product is photosensitive and should be protected from light.
- 储存条件
- 4 °C
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- 抗原
- IgA
- Abstract
- IgA 产品
- 物质类
- Antibody
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