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小鼠 anti-人 IgM Antibody (TRITC)

IF Monoclonal NI 179 IgG1 kappa TRITC
产品编号 ABIN457907
发货至: 中国
  • 抗原 See all IgM products
    IgM
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    小鼠
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    单克隆
    标记
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    TRITC
    应用范围
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    Immunofluorescence (IF)
    特异性
    The reactivity of the antiserum is restricted to a isotype specific determinant of the IgM molecule as tested in indirect binding enzyme immunoassay, immunoblotting, immuno-precipitation and direct immunofluorescence staining of cytoplasmic Ig. Tetramethylrhodamine isothiocyanate-conjugated purified monoclonal mouse antibody to human IgM, isotype specific
    产品特性
    Tetramethylrhodamine isothiocyanate-conjugated purified monoclonal mouse antibody to human IgM, isotype specific
    Fluorochrome/IgG protein molar ratio (E/P) approximately 5.7.
    纯化方法
    Purified
    免疫原
    Highly purified IgM isolated from human serum.
    克隆位点
    NI 179
    亚型
    IgG1 kappa
  • 应用备注
    To identify the presence of IgM in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as direct and indirect immunofluorescence staining of cytoplasmic IgM and cell membrane IgM.
    The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:10 to 1:100. These data should be interpreted as general recommendations only.
    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    Reconstitute the lyophilized product by adding 0. 5 mL sterile distilled water.
    浓度
    0.4 mg/mL
    缓冲液
    Purified monoclonal mouse IgG1 kappa conjugated with TRITC, lyophilized from a solution in phosphate buffered saline (pH 7.2).
    储存液
    Without preservative
    注意事项
    Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7. 2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted ascites should be stored at +4 °C, not refrozen, and preferably used the same day.
    储存条件
    4 °C/-20 °C
    储存方法
    The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.
  • 抗原
    IgM
    Abstract
    IgM 产品
    物质类
    Antibody
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