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小鼠 anti-人 IgA2 (IgA2(m)2) Antibody (Biotin)

ELISA, WB, ICC, IHC (p), DB Monoclonal NI 194-3 (A89-040) IgG1 Biotin
产品编号 ABIN457865
发货至: 中国
  • 抗原 See all IgA2 products
    IgA2
    抗原表位
    • 4
    • 4
    • 1
    IgA2(m)2
    适用
    宿主
    • 11
    • 2
    小鼠
    克隆类型
    • 11
    • 2
    单克隆
    标记
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    Biotin
    应用范围
    • 10
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    ELISA, Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Dot Blot (DB)
    特异性
    Biotin-conjugated purified monoclonal Mouse antibody to Human IgA2(m)2, allotype specific
    交叉反应 (详细)
    The antiSerum does not react with any other component of the Human Ig system or any other Human plasma protein as tested. This antiSerum has not been tested for cross-reactivity with other species.
    产品特性
    The reactivity of the antiserum is restricted to an allotype specific determinant on IgA2(m)2 Molecule as tested in haemagglutination, haemagglutination inhibition, direct binding enzyme immunoassay, competitive inhibition enzyme immunoassay, immunoblotting, immunoprecipitation, latex agglutination assay and histochemistry (Results of an IUIS/WHO collaborative study, Mestecky J. et al. (1996) J. Immunol. Methods 193, 103-148). To identify the presence of IgA2(m)2 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, immunoperoxidase staining of cytoplasmic IgA2(m)2, and immunoblotting. As a second step an avidin or streptavidin conjugate of the customer's choice have to be used. The optimum working dilution is an assayrelated characteristic. It may vary widely and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200, in ELISA from 1:200 upwards, in Western blotting from 1:200 upwards.
    纯化方法
    Purified
    免疫原
    Highly purified monoclonal IgA2(m)2 isolated from human serum.
    克隆位点
    NI 194-3 (A89-040)
    亚型
    IgG1
  • 应用备注
    ELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting.
    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    Reconstitute the lyophilized product by adding 0.5 mL sterile distilled water.Purified monoclonal mouse IgG1 kappa conjugated with biotin, lyophilized from a solution in phosphate buffered saline ( pH 7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added. Reconstitute the lyophilized product by adding 0.5 mL sterile distilled water.
    缓冲液
    Purified monoclonal mouse IgG1 kappa conjugated with biotin, lyophilized from a solution in phosphate buffered saline ( pH 7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added.
    储存液
    Without preservative
    储存条件
    RT,4 °C,-20 °C
    储存方法
    The lyophilized product is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted solutions should be stored at +4°C, not refrozen, and preferably used the same day.
  • 抗原
    IgA2
    别名
    IgA22 (IgA2 产品)
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