小鼠 anti-人 IgA Antibody (Biotin)
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- 抗原 See all IgA products
- IgA
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- Biotin
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应用范围
- ELISA, Western Blotting (WB)
- 特异性
- The reactivity of the antiserum is restricted to determinants on the C H 2 domain of IgA. It reacts with both subclasses of IgA as tested in haemagglutination, haemagglutination inhibition, direct binding enzyme immunoassay, competitive inhibition enzyme immunoassay, immunoblotting, immunoprecipitation, latex agglutination assay and histochemistry (Results of an IUIS/WHO collaborative study, Mestecky J. et al. (1996) J. Immunol. Methods 193, 103-148). Biotin-conjugated purified monoclonal mouse antibody to human IgA, isotype specific
- 产品特性
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Biotin-conjugated purified monoclonal mouse antibody to human IgA, isotype specific
- 纯化方法
- Purified
- 免疫原
- Highly purified monoclonal IgA isolated from human serum.
- 克隆位点
- NI 69 (A89-034) and NI 184 (A89-035)
- 亚型
- IgG1 kappa
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Goat anti-Human IgA antibody (PE)
ELISA, FACS Polyclonal IgG PE F(ab')2 fragment
Goat anti-Human IgA antibody (HRP) - PreadsorbedELISA Polyclonal IgG HRP
Goat anti-Human IgA antibodyELISA, FACS Polyclonal IgG unconjugated F(ab')2 fragment
Goat anti-Human IgA (Heavy Chain) antibody (Biotin)ELISA, WB, FACS Polyclonal IgG Biotin
Goat anti-Human IgA (Heavy Chain) antibody (FITC)ELISA, FACS Polyclonal IgG FITC
Goat anti-Human IgA (Heavy Chain) antibody (TRITC)ELISA, FACS Polyclonal IgG TRITC
Goat anti-Human IgA antibody (Biotin)ELISA, FACS Polyclonal IgG Biotin F(ab')2 fragment
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- 应用备注
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To identify the presence of IgA in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, immunoperoxidase staining of cytoplasmic IgA, and immunoblotting. As a second step an avidin or streptavidin conjugate of the customer's choice have to be used.
The optimum working dilution is an assay-related characteristic. It may vary widely and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200, in ELISA from 1:250 upwards, in Western blotting from 1:500 upwards. Working dilutions may vary widely, strongly depending on the test conditions. These data should be interpreted as general recommendations only. - 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Reconstitute the lyophilized product by adding 0. 5 mL sterile distilled water.
- 浓度
- 0.4 mg/mL
- 缓冲液
- Purified monoclonal mouse IgG1 kappa conjugated with biotin, lyophilized from a solution in phosphate buffered saline (pH 7.2).
- 储存液
- Without preservative
- 注意事项
- Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7. 2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted ascites should be stored at +4 °C, not refrozen, and preferably used the same day.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.
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- 抗原
- IgA
- Abstract
- IgA 产品
- 物质类
- Antibody
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