猪 anti-Guinea 猪 IgG (Heavy & Light Chain) Antibody
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- 抗原 See all IgG products
- IgG
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抗原表位
- Heavy & Light Chain
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适用
- 豚鼠
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宿主
- 猪
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克隆类型
- 多克隆
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应用范围
- ELISA, Western Blotting (WB), Immunofluorescence (IF), Dot Blot (DB)
- 特异性
- Purified IgG fraction of polyclonal Swine antiSerum to Guinea Pig IgG, heavy and light chains.
- 交叉反应 （详细）
- Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail.
- 产品特性
- The reactivity of the antiserum is directed to the Fc and Fab subunits of the IgG molecule. It includes a certain degree of reactivity with other immunoglobulins via the common Fab portion. It does not react with any non-Ig protein in guinea pig serum, as tested by immunoelectrophoresis and double radial immunodiffusion. As unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user's own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration.
- 纯化方法
- Purified
- 免疫原
- Purified normal IgG isolated from pooled guinea pig serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
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Goat anti-Guinea Pig IgG (Heavy & Light Chain) antibody (HRP) - Preadsorbed
ELISA, IHC, WB, DB, IEM Polyclonal IgG HRP
Donkey anti-Guinea Pig IgG (Heavy & Light Chain) antibody - PreadsorbedELISA, WB Polyclonal IgG unconjugated
Goat anti-Guinea Pig IgG (Heavy & Light Chain) antibody (HRP) - PreadsorbedELISA, WB Polyclonal IgG HRP
Rabbit anti-Guinea Pig IgG (Heavy & Light Chain) antibody (HRP)ELISA Polyclonal IgG HRP
Donkey anti-Guinea Pig IgG (Heavy & Light Chain) antibody (Biotin) - PreadsorbedELISA, IHC, WB Polyclonal IgG Biotin
Goat anti-Guinea Pig IgG (Fc Region) antibody (Alkaline Phosphatase (AP)) - PreadsorbedELISA, IHC, WB Polyclonal IgG Alkaline Phosphatase (AP)
Goat anti-Guinea Pig IgG (Heavy & Light Chain) antibody (Alkaline Phosphatase (AP)) - PreadsorbedELISA, IHC, WB Polyclonal IgG Alkaline Phosphatase (AP)
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- 应用备注
- Indirect immunofluorescence,ELISA,Dot blot,Immunoblotting.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C.Purified hyperimmune swine IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added. It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C.
- 缓冲液
- Purified hyperimmune swine IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added.
- 储存液
- Without preservative
- 储存条件
- RT,4 °C,-20 °C
- 储存方法
- The lyophilized IgG fraction is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
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- 抗原
- IgG
- Abstract
- IgG 产品
- 物质类
- Antibody
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