山羊 anti-小鸡 IgM (Fc Region) Antibody (Biotin)
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- 抗原 See all IgM products
- IgM
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抗原表位
- Fc Region
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适用
- 小鸡
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- Biotin
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应用范围
- ELISA, Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Dot Blot (DB)
- 特异性
- Biotin-conjugated IgG fraction of polyclonal Goat antiSerum to Chicken IgM, Fc specific
- 交叉反应 （详细）
- Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this immunoconjugate has not been tested in detail.
- 产品特性
- The reactivity of the antiserum is directed to the Fc subunit of the IgM molecule which expresses strict isotypic (class) specificity. It does not react with IgG, IgA and IgG/Fab or any non-Ig protein in chicken serum, as tested by immunoelectrophoresis and double radial immunodiffusion. In immunocytochemical and immunohistochemical staining of IgM at the cellular and subcellular level of appropriately treated cell and tissue substrates, to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases, to identify a specific antigen using a reference antibody of chicken origin known to be of the IgM isotype in the middle layer of the indirect test procedure, in non-isotopic assay methodology (e.g. ELISA) to measure IgM in chicken serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user's choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
- 纯化方法
- Purified
- 免疫原
- Purified IgM isolated from chicken serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
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Goat anti-Chicken IgM antibody - Preadsorbed
ELISA, IHC, WB Polyclonal IgG unconjugated
Mouse anti-Chicken IgM antibody (PE)ELISA, FACS Monoclonal M-1 IgG2b PE
Mouse anti-Chicken IgM antibody (SPRD)ELISA, FACS Monoclonal M-1 IgG2b SPRD
Goat anti-Chicken IgM antibody (Biotin) - PreadsorbedELISA, IHC, WB Polyclonal IgG Biotin
Goat anti-Chicken IgM (Chain mu) antibody (HRP)ELISA Polyclonal IgG HRP
Goat anti-Chicken IgM antibody (Alkaline Phosphatase (AP)) - PreadsorbedELISA, IHC, WB Polyclonal IgG Alkaline Phosphatase (AP)
Mouse anti-Chicken IgM antibody (Biotin)ELISA, FACS Monoclonal M-1 IgG2b Biotin
Mouse anti-Chicken IgM antibody (APC)ELISA, FACS, FLISA Monoclonal M-1 IgG2b APC
Mouse anti-Chicken IgM antibody (FITC)ELISA, FACS Monoclonal M-1 IgG2b FITC
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- 应用备注
- ELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C.Biotin-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity. It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C.
- 缓冲液
- Biotin-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.
- 储存液
- Without preservative
- 储存条件
- RT,4 °C,-20 °C
- 储存方法
- The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.
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- 抗原
- IgM
- Abstract
- IgM 产品
- 物质类
- Antibody
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