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- 抗原 See all IgM products
- IgM
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适用
- Pig
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宿主
- 小鼠
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克隆类型
- 单克隆
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应用范围
- Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)
- 交叉反应 （详细）
- Species reactivity (tested):Porcine.Minimal Cross Reaction with Rat, Goat, Sheep, Donkey, Human (all <0.1 %) and Rabbit (0.1 %).
- 纯化方法
- Affinity Chromatography
- 免疫原
- Porcine IgM.
- 克隆位点
- 206-2
- 亚型
- IgG2b
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- 应用备注
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ELISA: 0.1 μg/mL (1/4000). Immunoprecipitation. Immunohistochemistry on Frozen Sections: 0.2 μg/mL (1/2000). Immunohistochemistry on Paraffin Sections (Does not react on routinely processedParaffin Section). Does not react in Western Blot with reduced and denatured samples.
Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 溶解方式
- Restore with 0.5 mL distilled water to make stock solution.
- 浓度
- 0.4 mg/mL
- 缓冲液
- PBS, pH 7.2 containing 5 mg/mL BSA as a stabilizer and 0.09 % Sodium Azide as preservative.
- 储存液
- Sodium azide
- 注意事项
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- 注意事项
- Avoid repeated thawing and freezing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store lyophilized product at 2-8 °C. Store stock solution or aliquots thereof at -28 °C.
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- 抗原
- IgM
- Abstract
- IgM 产品
- 物质类
- Antibody
- 背景
- IgM antibodies form a major proportion of the primary antibody response, whereas the secondary response consists almost entirely of IgG. IgMs are often referred to as macroglobulins because of their high molecular weight. They form a pentameric structure consisting of five subunits and a single J-chain. Each subunit contains the well-known pairs of heavy and light chains, with the particular feature of the heavy (μ) chain containing one variable and four constant regions. The molecular weight of intact IgM is approximately 900 kDa. In polyacrylamide gel electrophoresis under denaturing conditions, the heavy chain migrates as a band of approximately 85 kDa.Synonyms: Pig Immunoglobulin M
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