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小鼠 anti-人 IgG (Fc Region) Antibody (PE)

ELISA, FACS Monoclonal JDC-10 IgG1 PE
产品编号 ABIN376566
发货至: 中国
  • 抗原 See all IgG products
    IgG
    抗原表位
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    Fc Region
    适用
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    小鼠
    克隆类型
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    • 1
    单克隆
    标记
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    PE
    应用范围
    • 1952
    • 1837
    • 1807
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    • 478
    • 380
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    ELISA, Flow Cytometry (FACS)
    特异性
    Reacts with the Fc portion of the heavy chain of all subclasses of human IgG as demonstrated by ELISA,
    产品特性
    Mouse Anti-Human IgG Fc-PE
    纯化方法
    Purified
    免疫原
    Purified human IgG1 myeloma protein
    克隆位点
    JDC-10
    亚型
    IgG1
  • 应用备注
    • Applications: ELISA - Quality tested , FLISA - Quality tested FC - Quality tested , IHC-PS - Reported in literature , WB - Reported in literature , Multiplex - Reported in literature
    • Working Dilutions: ELISA AP conjugate 1:1,000 - 1:4,000 HRP conjugate 1:2,000 - 1:8,000 BIOT conjugate 1:5,000 - 1:10,000 FLISA AF488 conjugate 1:200 - 1:400 PE and AF647 conjugates 1 g/mL Flow Cytometry Purified (UNLB) antibody 1 g/106 cells BIOT and AF488 conjugates 1 g/106 cells PE and AF647 conjugates 0.2 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L
    限制
    仅限研究用
  • 浓度
    0.1 mg/mL
    缓冲液
    0.1 mg in 1.0 mL of PBS/Sodium azide and a stabilizing agent
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Do not freeze!
    Protect conjugated products from light.

    Each reagent is stable for the period shown on the bottle label if stored as directed.
    储存条件
    4 °C
    储存方法
    Store at 2-8°C
  • Gilman, Furmanova-Hollenstein, Pascual, B van t Wout, Langedijk, McLellan: "Transient opening of trimeric prefusion RSV F proteins." in: Nature communications, Vol. 10, Issue 1, pp. 2105, (2019) (PubMed).

    Chung, Nguyen, Lin, Lafrance-Vanasse, Scales, Lin, Deng, Williams, Sperinde, Li, Zheng, Sukumaran, Tesar, Ernst, Fischer, Lazar, Prabhu, Song: "An in vitro FcRn- dependent transcytosis assay as a screening tool for predictive assessment of nonspecific clearance of antibody therapeutics in humans." in: mAbs, Vol. 11, Issue 5, pp. 942-955, (2019) (PubMed).

    Boesch, Kappel, Mahan, Chu, Crowley, Osei-Owusu, Alter, Ackerman: "Enrichment of high affinity subclasses and glycoforms from serum-derived IgG using FcγRs as affinity ligands." in: Biotechnology and bioengineering, Vol. 115, Issue 5, pp. 1265-1278, (2019) (PubMed).

    Brown, Dowell, Boesch, Normandin, Mahan, Chu, Barouch, Bailey-Kellogg, Alter, Ackerman: "Multiplexed Fc array for evaluation of antigen-specific antibody effector profiles." in: Journal of immunological methods, Vol. 443, pp. 33-44, (2017) (PubMed).

    Brown, Normandin, Osei-Owusu, Mahan, Chan, Lai, Vaccari, Rao, Franchini, Alter, Ackerman: "Microscale purification of antigen-specific antibodies." in: Journal of immunological methods, Vol. 425, pp. 27-36, (2016) (PubMed).

    Brown, Licht, Dugast, Choi, Bailey-Kellogg, Alter, Ackerman: "High-throughput, multiplexed IgG subclassing of antigen-specific antibodies from clinical samples." in: Journal of immunological methods, Vol. 386, Issue 1-2, pp. 117-23, (2013) (PubMed).

  • 抗原
    IgG
    Abstract
    IgG 产品
    物质类
    Antibody
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