Applications: Quality tested applications include - ELISA , FLISA FC ,
Other referenced applications include - ELISPOT , IHC-FS , Stim
Working Dilutions: ELISA BIOT conjugate 1:5,000 - 1:20,000 FLISA FITC conjugate 1:100 - 1:400 AF555 conjugate 1:200 - 1:400 PE and AF647 conjugates 1 g/mL Flow Cytometry FITC and BIOT conjugates 1 g/106 cells PE and AF647 conjugates 0.1 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L
限制
仅限研究用
缓冲液
0.25 mg in 1.0 mL of PBS/Sodium azide and a stabilizing agent
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Do not freeze! Protect conjugated products from light. Each reagent is stable for the period shown on the bottle label if stored as directed.
储存条件
4 °C
储存方法
Store at 2-8°C
Brandsma, Bondza, Evers, Koutstaal, Nederend, Jansen, Rösner, Valerius, Leusen, Ten Broeke: "Potent Fc Receptor Signaling by IgA Leads to Superior Killing of Cancer Cells by Neutrophils Compared to IgG." in: Frontiers in immunology, Vol. 10, pp. 704, (2019) (PubMed).
Magri, Comerma, Pybus, Sintes, Lligé, Segura-Garzón, Bascones, Yeste, Grasset, Gutzeit, Uzzan, Ramanujam, van Zelm, Albero-González, Vazquez, Iglesias, Serrano, Márquez, Mercade, Mehandru, Cerutti: "Human Secretory IgM Emerges from Plasma Cells Clonally Related to Gut Memory B Cells and Targets Highly Diverse Commensals." in: Immunity, Vol. 47, Issue 1, pp. 118-134.e8, (2017) (PubMed).