ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
特异性
Reacts with the heavy chain of mouse IgG1 as demonstrated by ELISA and flow cytometry.
产品特性
Source: Pooled antisera from goats hyperimmunized with mouse IgG 1 paraproteins. To insure lot-to-lot consistency, each batch of product is tested by ELISA, Fluorescent ELISA, and/or flow cytometry to conform to characteristics of a standard reference reagent.
纯化方法
Affinity chromatography on mouse IgG1 covalently linked to agarose. Cross Absorption: Mouse IgM, IgG2a, IgG2b, IgG3 and IgA, pooled human sera and purified human paraproteins.
Working Dilution: ELISA: 1:4,000-1:8,000 Indirect immunostaining in conjunction with mouse IgG1 monoclonal antibodies. Representative data are included in this product insert.
Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
限制
仅限研究用
状态
Liquid
注意事项
Avoid repeated freezing and thawing. Dilute only prior to immediate use Centrifuge product if not completely clear after standing at room temperature. Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
储存条件
4 °C/-20 °C
储存方法
Store at 2-8 °C or long-term at -20 °C.
Margry, Kersemakers, Hoek, Arkesteijn, Wieland, van Eden, Broere: "Activated peritoneal cavity B-1a cells possess regulatory B cell properties." in: PLoS ONE, Vol. 9, Issue 2, pp. e88869, (2014) (PubMed).
Stegmann, Kamphuis, Meijerhof, Goud, de Haan, Wilschut: "Lipopeptide-adjuvanted respiratory syncytial virus virosomes: A safe and immunogenic non-replicating vaccine formulation." in: Vaccine, (2010) (PubMed).