ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Flow Cytometry (FACS)
特异性
Reacts with the heavy and light chains of rat IgG 1, IgG 2a, IgG 2b and IgG 2c, and with the light chains of rat IgM as demonstrated by ELISA and/or flow cytometry.
产品特性
Source: Pooled antisera from rabbits hyperimmunized with rat IgG. To insure lot-to-lot consistency, each batch of product is tested by ELISA, PCFIA and/or flow cytometry for conformance to characteristics of a standard reference reagent.
纯化方法
Affinity chromatography on sheep IgG covalently linked to agarose.
Working Dilution: <= 1 µg/10^6 cells or 1:5,000-1:20,000 Representative data are included in this product insert.
Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.