ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Flow Cytometry (FACS)
特异性
Reacts with the heavy and light chains of rabbit IgG, and with the light chains of rabbit IgM and IgA as demonstrated by ELISA and flow cytometry.
产品特性
Source: Pooled antisera from goats hyperimmunized with normal rabbit IgG. To insure lot-to-lot consistency, each batch of product is tested by ELISA, PCFIA and/or flow cytometry for conformance to characteristics of a standard reference reagent.
纯化方法
Affinity chromatography on human IgM covalently linked to Sepharose-4B.
Working Dilution: <= 1 µg/10^6 cells or 1:5,000-1:20,000 Representative data are included in this product insert.
Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.