小鼠 anti-大鼠 IgM Antibody (FITC)
Quick Overview for 小鼠 anti-大鼠 IgM Antibody (FITC) (ABIN371245)
抗原
克隆类型
标记
应用范围
克隆位点
亚型
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适用
- 大鼠
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宿主
- 小鼠
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Mouse anti-Rat IgM antibody
ELISA, FACS Monoclonal M2A1 IgG1 unconjugated
Mouse anti-Rat IgM antibody (PE)ELISA, FACS Monoclonal M2A1 IgG1 PE
Mouse anti-Rat IgM antibody (FITC)ELISA, FACS Monoclonal M2A1 IgG1 FITC
Mouse anti-Rat IgM antibody (HRP)ELISA, FACS, IC Monoclonal M 2A1 IgG1 HRP
Mouse anti-Rat IgM antibody (Cy5)ELISA, FACS Monoclonal M2A1 IgG1 Cy5
Mouse anti-Rat IgM antibodyELISA, FACS Monoclonal M2A1 IgG1 unconjugated F(ab')2 fragment
Goat anti-Rat IgM (Heavy Chain) antibodyELISA, FACS, IHC, WB Polyclonal IgG unconjugated
Goat anti-Rat IgM (Heavy Chain) antibody (Texas Red (TR))ELISA, FACS, IHC Polyclonal IgG Texas Red (TR)
Goat anti-Rat IgM (Heavy Chain) antibody (TRITC)ELISA, FACS, IHC Polyclonal IgG TRITC
Goat anti-Rat IgM (Heavy Chain) antibody (FITC)ELISA, FACS, IHC Polyclonal IgG FITC
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应用备注
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Flow Cytometry: < / = 0.3 μg/10^6 cells. Immunofluorescence Microscopy: Identification and enumeration of IgM+ cells.
Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user. -
说明
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Excitation/Emission wavelength: 494 nm/514 nm
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限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- PBS containing 0.09 % Sodium Azide as preservative.
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储存液
- Sodium azide
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注意事项
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
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注意事项
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Avoid repeated freezing and thawing.
This product is photosensitive and should be protected from light. -
储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or in (aliquots) at -28 °C for longer.
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- IgM
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物质类
- Antibody
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背景
- IgM normally constitutes about 10 % of serum immunoglobulins. IgM antibody is prominent in early immune responses to most antigens and predominates in certain antibody responses such as 'natural' blood group antibodies. IgM (with IgD) is the major immunoglobulin expressed on the surface of B cells. The gene for the mu constant region contains four domains separated by short intervening sequences. Class specific anti immunoglobulin antibodies are useful for: The characterization of malignant B cell proliferations. All but acute lymphocytic leukemias share either surface or intra cytoplasmic Ig with an isotypic restriction, which suggest the monoclonal nature of the cell population. Most of the chronic lymphocytic leukemias, non Hodgkin lymphomas and Burkitt's lymphoma bear surface IgM, whereas plasmocytes from Waldenström's disease bear intracytoplasmic IgM. The other isotypes are less frequently found. On the other hand multiple myelomas are usually of the IgG or IgA type. Characterization of plasma cells in inflammatory conditions: Plasma cell typing can be of use for the classification of intestinal inflammatory conditions such as inflammatory bowel disease and allergic conditions. In the latter a specific increase in the number of IgE plasma cells can be demonstrated.Synonyms: Rat Immunoglobulin M
抗原
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