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山羊 anti-兔 IgG Antibody (Alkaline Phosphatase (AP))

ELISA, IHC, WB, ICC, IA Polyclonal Alkaline Phosphatase (AP)
产品编号 ABIN336728
发货至: 中国
  • 抗原 See all IgG products
    IgG
    适用
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    山羊
    克隆类型
    • 3434
    • 175
    • 1
    多克隆
    标记
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    Alkaline Phosphatase (AP)
    应用范围
    • 1953
    • 1837
    • 1807
    • 1520
    • 934
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    • 478
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    ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Immunocytochemistry (ICC), Immunoassay (IA)
    纯化方法
    Affinity purified using solid phase Rabbit IgG
    免疫原
    Goat serum was obtained from animals of US origin and under the care of a registered veterinarian.
  • 应用备注
    This conjugate is suitable for all immunoassay applications.
    The optimal working dilution should be determined by the investigator. Suggested starting dilution: 1:500-1:2,000 for Immunohistochemistry, 1:500- 1:8,000 for ELISA/Western blot
    说明

    ALP: Enzyme derived from calf intestine (U.S. origin), Catalyzes the hydrolysis of phosphate groups from a substrate, which produces a colored reaction product or a release of light, The most common substrate for ALP is pNPP, which produces a soluble product, Unaffected by biological components in the ELISA assay, and produces consistent results, The reaction rate of ALP is linear, so its detection sensitivity can be increased by increasing the length of the reaction time. Detection OD of 405 nm

    限制
    仅限研究用
  • 缓冲液
    30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free. Preservative: 0.05 % (w/v) Sodium Azide
    储存液
    Sodium azide
    注意事项
    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
    注意事项
    Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
    Do not add Sodium azide.
    Dilute only prior to immediate use
    Each reagent is stable for the period shown on the bottle label if stored as directed.
    储存条件
    4 °C
  • 抗原
    IgG
    Abstract
    IgG 产品
    物质类
    Antibody
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