山羊 anti-人 IgE Antibody (Alkaline Phosphatase (AP))
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- 抗原 See all IgE products
- IgE
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适用
- 人
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- Alkaline Phosphatase (AP)
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应用范围
- ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoassay (IA)
- 纯化方法
- Affinity purified using solid phase Human IgE
- 免疫原
- Goat serum was obtained from animals of US origin and under the care of a registered veterinarian.
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Mouse anti-Human IgE antibody
ELISA, FACS Monoclonal 4G7-325 IgG unconjugated
Mouse anti-Human IgE (Fc Region) antibody (Biotin)ELISA Monoclonal HP6029 IgG2a Biotin
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- 应用备注
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This conjugate is suitable for all immunoassay applications.
The optimal working dilution should be determined by the investigator. Suggested starting dilution: 1:500-1:2,000 for ELISA/Western blot 1:20. 1:2,000 for Immunohistochemistry 1:50. 1:5,000 for Immunocytochemistry - 说明
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ALP: Enzyme derived from calf intestine (U.S. origin), Catalyzes the hydrolysis of phosphate groups from a substrate, which produces a colored reaction product or a release of light, The most common substrate for ALP is pNPP, which produces a soluble product, Unaffected by biological components in the ELISA assay, and produces consistent results, The reaction rate of ALP is linear, so its detection sensitivity can be increased by increasing the length of the reaction time. Detection OD of 405 nm
- 限制
- 仅限研究用
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- 缓冲液
- 30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free. Preservative: 0.05 % (w/v) Sodium Azide
- 储存液
- Sodium azide
- 注意事项
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- 注意事项
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Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Do not add Sodium azide.
Dilute only prior to immediate use
Each reagent is stable for the period shown on the bottle label if stored as directed. - 储存条件
- 4 °C
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- 抗原
- IgE
- Abstract
- IgE 产品
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