ELISA, WB, IHC, ICC, IA
Polyclonal
Alkaline Phosphatase (AP)
应用备注
ELISA: 1/1000-1/10000. Western Blot: 1/1000-1/5000.
Other applications not tested. Otimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
浓度
0.5 mg/mL
缓冲液
50 mM HEPES containing 0.1 M NaCl, 1 mM MgCl2, 0.1 mM ZnCl2 with 0.09 % Sodium Azide as preservative and 0.2 % BSA as stabilizer.
储存液
Sodium azide
注意事项
WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
注意事项
Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. Do not add Sodium azide. Dilute only prior to immediate use Each reagent is stable for the period shown on the bottle label if stored as directed.