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小鼠 anti-人 IgM Heavy Chain Antibody

IHC (p), WB, FACS, IF Monoclonal IM373 IgG1 kappa unconjugated
产品编号 ABIN3026664
发货至: 中国
  • 抗原
    IgM Heavy Chain
    适用
    宿主
    • 1
    小鼠
    克隆类型
    • 1
    单克隆
    应用范围
    Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF)
    产品特性
    This antibody recognizes a protein of 75 kDa, identified as the mu heavy chain of human immunoglobulins. It does not cross-react with alpha (IgA), gamma (IgG), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. The antibody is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.
    纯化方法
    Protein G affinity chromatography
    免疫原
    Recombinant heavy chain of human IgM was used as the immunogen for this antibody.
    克隆位点
    IM373
    亚型
    IgG1 kappa
  • 应用备注
    The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/million cells,IF: 0.5-1 μg/mL,WB: 0.5-1 μg/mL,IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2)

    限制
    仅限研究用
  • 浓度
    0.2 mg/mL
    缓冲液
    0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Store the IgM heavy chain antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
  • 抗原
    IgM Heavy Chain
    背景
    This antibody recognizes a protein of 75  kDa, identified as the mu heavy chain of human immunoglobulins. It does not cross-react with alpha (IgA), gamma (IgG), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. The antibody is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.
    基因ID
    3507
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