绵羊 anti-人 IgG (Whole Molecule) Antibody (Alkaline Phosphatase (AP))
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- 抗原 See all IgG products
- IgG
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抗原表位
- Whole Molecule
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适用
- 人
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宿主
- 绵羊
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克隆类型
- 多克隆
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标记
- Alkaline Phosphatase (AP)
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应用范围
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- 特异性
- Anti-Alkaline Phosphatase (calf intestine), anti-Sheep Serum, Human IgG and Human Serum
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Human IgG whole molecule.
Type of Immunogen: Purified protein
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- 应用备注
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Approved: ELISA (1:2000 - 1:8000), IHC, WB
Usage: Suitable for immunoblotting (western or dot blot), ELISA and immunohistochemistry as well as other phosphatase-anti body based enzymatic assays requiring lot-to-lot consistency. This product has been assayed against 1.0 μg of Human IgG in a standard capture ELISA using pNPP (p-nitrophenyl phosphate catalog no. NPP-10) as a substrate for 30 minutes at room temperature. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. - 说明
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Target Species of Antibody: Human
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- 0.05 M Tris chloride, 0.15 M sodium chloride, 0.001 M magnesium chloride, 0.0001 M zinc chloride, 50 % glycerol, pH 8.0, 10 mg/mL BSA (IgG, protease free), 0.01 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
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Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Do not add Sodium azide.
Dilute only prior to immediate use
Each reagent is stable for the period shown on the bottle label if stored as directed. - 储存条件
- 4 °C
- 储存方法
- Store at 4°C. Do not freeze.
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- 抗原
- IgG
- Abstract
- IgG 产品
- 物质类
- Antibody
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