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山羊 anti-兔 IgG (Heavy & Light Chain) Antibody (FITC) - Preadsorbed

Rockland 611-102-122 WB, FACS, FLISA, FM, DB Polyclonal IgG FITC
Rockland
产品编号 ABIN2669863
Supplier Product No.: 611-102-122
发货至: 中国
  • 抗原 See all IgG products
    IgG
    抗原表位
    • 2402
    • 511
    • 417
    • 148
    • 129
    • 128
    • 67
    • 29
    • 28
    • 28
    • 21
    • 9
    • 5
    • 5
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    • 1
    Heavy & Light Chain
    适用
    • 720
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    • 1
    • 1
    • 1
    宿主
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    • 11
    山羊
    克隆类型
    • 3389
    • 175
    • 1
    多克隆
    标记
    • 583
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    FITC
    应用范围
    • 2033
    • 1982
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    Western Blotting (WB), Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM), Dot Blot (DB)
    Supplier Product No.
    611-102-122
    Supplier
    Rockland
    原理
    Rabbit IgG (H&L) Secondary Antibody Fluorescein Conjugated Pre-Adsorbed
    交叉反应 (详细)
    Minimal crossreactivity against Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Rabbit IgG and Rabbit Serum.  No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat or Sheep Serum Proteins.
    产品特性
    Anti-Rabbit IgG (H&L) Antibody generated in goat detects reactivity to Rabbit IgG.
    纯化方法
    Secondary antibody conjugate was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
    免疫原
    Optional[Immunogen]: Rabbit IgG whole molecule
    亚型
    IgG
  • 应用备注
    Application Note: Anti-Rabbit IgG Fluorescein Conjugate has been tested by dot blot and western blot. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment. Flow Cytometry Dilution: 1:500 - 1:2,500 FLISA Dilution: 1:10,000 - 1:50,000 IF Microscopy Dilution: 1:1,000 - 1:5,000
    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL
    浓度
    1.0 mg/mL
    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    , Preservative:0.01 % (w/v) Sodium Azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Store secondary antibody at 4° C prior to restoration.   For extended storage aliquot antibody and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
    有效期
    12 months
  • Warburton, Redmond, Dooley, Fu, Gillison, Akagi, Symer, Aladjem, McBride: "HPV integration hijacks and multimerizes a cellular enhancer to generate a viral-cellular super-enhancer that drives high viral oncogene expression." in: PLoS genetics, Vol. 14, Issue 1, pp. e1007179, (2018) (PubMed).

  • 抗原
    IgG
    Abstract
    IgG 产品
    物质类
    Antibody
    背景
    Anti-Rabbit secondary antibody conjugated to FITC is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
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