Usage: The reactivity of the antiserum is restricted to a subclass specific determinant on the Fc part of the IgG1 Molecule as tested in indirect binding enzyme immunoassay, immunoblotting, immunoprecipitation and indirect immunoperoxidase staining of cytoplasmic Ig To identify the presence of IgG1 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase and indirect immunofluorescence staining of cytoplasmic IgG1, and immunoblotting. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200. in ELISA from 1:500 upwards. in Western blotting from 1:1000 upwards. These data should be interpreted as general recommendations only.
限制
仅限研究用
状态
Lyophilized
溶解方式
Distilled water
浓度
Lot specific
缓冲液
Lyophilized from PBS, pH 7.2. No preservative added.
储存液
Without preservative
注意事项
Avoid repeat freeze-thaw cycles.
储存条件
4 °C,-20 °C
储存方法
Long term: -20°C Short term: +4°C. Avoid repeat freeze-thaw cycles.