Immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as otherantibodybased assays using streptavidin or avidin conjugates: ELISA: 1/60,000-1/600,000 Western Blot: 1/2,000-1/10,000Immunohistochemistry: 1/1,000-1/5,000
Other applications not tested. Otimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
溶解方式
Restore with 1.0 mL of deionized water
浓度
1.0 mg/mL
缓冲液
0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, containg 10 mg/mL BSA (IgG and Protease free) and 0.01 % (w/v) Sodium Azide.
储存液
Sodium azide
注意事项
WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
注意事项
Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
储存条件
4 °C/-20 °C
储存方法
Store vial at 2-8 °C prior to restoration. For extended storage after reconstitution, mix product with glycerol to 50 % final, then aliquot contents and freeze at -28 °C or below. This product is stable for one month at 2-8 °C as an undiluted liquid.