小鼠 anti-人 IgM Antibody
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- 抗原 See all IgM products
- IgM
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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应用范围
- ELISA, Western Blotting (WB)
- Supplier Product No.
- 209-301-031
- Supplier
- Rockland
- 原理
- Human IgM Fc5u Antibody
- 交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum, Human IgM, Human Serum and Human Fc5μ. No reaction was observed against Human IgG, Human IgA, Human Fc, Human Fab or Human Fab2.
- 产品特性
- Anti-Human IgG F(c) generated in mouse detects Human F(c). A proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme papain under controlled conditions of temperature, time and pH .
- 纯化方法
- Anti-Human IgM Fc5μ Antibody was prepared from concentrated roller bottle supernatant by Protein A chromatography sepharose beads.
- 过滤
- Sterile filtered
- 免疫原
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Immunogen: Anti-Human IgM Fc5mu was produced by repeated immunization with Human IgM Fc5mu fragment in mice.
Immunogen Type: Native Protein
- 克隆位点
- 22G1-H8
- 亚型
- IgG2b
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Mouse anti-Human IgM antibody (HRP)
ELISA, WB Monoclonal CH2 IgG1 HRP
Goat anti-Human IgM antibody (Biotin)ELISA, FACS Polyclonal IgG Biotin F(ab')2 fragment
Goat anti-Human IgM antibody (FITC)ELISA, FACS Polyclonal IgG FITC F(ab')2 fragment
Goat anti-Human IgM (Heavy Chain) antibody (TRITC)ELISA, FACS, IHC Polyclonal IgG TRITC
Goat anti-Human IgM (Heavy Chain) antibody (FITC)ELISA, FACS, IHC Polyclonal IgG FITC
Goat anti-Human IgM (Heavy Chain) antibody (Texas Red (TR))ELISA, FACS, IHC Polyclonal IgG Texas Red (TR)
Goat anti-Human IgM antibody (HRP) - PreadsorbedELISA, FLISA Polyclonal IgG HRP
Goat anti-Human IgM (Heavy Chain) antibody (Alkaline Phosphatase (AP))ELISA, IHC, WB Polyclonal IgG Alkaline Phosphatase (AP)
Goat anti-Human IgM antibody (HRP)ELISA, FACS, FLISA Polyclonal IgG HRP F(ab')2 fragment
Mouse anti-Human IgM (Heavy Chain) antibody (Alkaline Phosphatase (AP))ELISA, IHC, WB Monoclonal SA-DA4 IgG1 Alkaline Phosphatase (AP)
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- 应用备注
- Application Note: Human IgM Fc5μ antibody has been tested by ELISA and Western Blot. This product is suitable for immunoblotting (western or dot blot), ELISA, and immunohistochemistry requiring extremely low background levels, lot-to-lot consistency, high titer and specificity. Western Blot Dilution: 1:1000-1:2000 ELISA Dilution: 1:1000 - 1:10000
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
, Preservative:0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- IgM
- Abstract
- IgM 产品
- 物质类
- Antibody
- 背景
- Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together. Due to this large size, IgM is typically isolated to the serum. Anti-Human IgM Fc5μ antibody is ideal for investigators in Immunology, Microbiology, and Cell Biology.
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