小鼠 anti-人 IgM (Fc5mu Region) Antibody
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- 抗原 See all IgM products
- IgM
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抗原表位
- Fc5mu Region
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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应用范围
- ELISA, Western Blotting (WB)
- 特异性
- IgM Fc5µ
- 交叉反应
- 人
- 纯化方法
- Anti-Human IgM Fc5μ Antibody was prepared from concentrated roller bottle supernatant by Protein A chromatography sepharose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum, Human IgM, Human Serum and Human Fc5μ. No reaction was observed against Human IgG, Human IgA, Human Fc, Human Fab or Human Fab2.
- 免疫原
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Immunogen: Anti-Human IgM Fc5mu was produced by repeated immunization with Human IgM Fc5mu fragment in mice.
Immunogen Type: Native Protein
- 克隆位点
- 22G1-H8
- 亚型
- IgG2b kappa
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- 应用备注
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Application Note: Human IgM Fc5μ antibody is suitable for immunoblotting (western or dot blot), ELISA, and immunohistochemistry requiring extremely low background levels, lot-to-lot consistency, high titer and specificity.
ELISA Dilution: 1:1000-1:10000
Western Blot Dilution: 1:1000-1:2000
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
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Avoid cycles of freezing and thawing.
Centrifuge product if not completely clear after standing at room temperature. - 储存条件
- RT,4 °C,-20 °C
- 储存方法
- Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. This product is stable for several weeks at 4 °C as an undiluted liquid.
- 有效期
- 12 months
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- 抗原
- IgM
- Abstract
- IgM 产品
- 物质类
- Antibody
- 背景
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Synonyms: mouse anti-Human IgM Fc5μ Antibody, Human IgM Fc5u Antibody, Ms-a-Human IgM Fc5μ Antibody, Human IgM Fc5μ Antibody in Mouse, Human M Fc5μ Secondary Antibody
Background: Anti-Human IgM Fc5μ antibody generated in mice specifically detects Fc5μ portion of the human IgM heavy chain. Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together. Due to this large size, IgM is typically isolated to the serum. Anti-Human IgM Fc5μ antibody is ideal for investigators in Immunology, Microbiology, and Cell Biology.
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