After multiple immunizations in Freunds adjuvant, eggs were collected and the IgY fraction prepared. The antibodies were immunoaffinity purified from a solid phase immunoabsorbent and conjugated to horseradish peroxidase (HRP).
纯化方法
egg yolk-derived purified IgY, affinity purified
免疫原
Chickens were immunized with highly purified rabbit IgG isolated from normal pooled rabbit serum.
Optimal working dilutions should be determined for your particular assay conditions.
限制
仅限研究用
状态
Liquid
浓度
1.0 mg/mL
缓冲液
Phosphate buffered saline, 50 % glycerol
储存液
Thimerosal (Merthiolate)
注意事项
Biohazard Informations: This product contains thimerosal which is hazardous.
注意事项
Avoid repeated freezing and thawing. Dilute only prior to immediate use Centrifuge product if not completely clear after standing at room temperature. Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.