After multiple immunizations in Freunds adjuvant, eggs were collected and the IgY fraction prepared. The antibodies were immunoaffinity purified from a solid phase immunoabsorbent and conjugated to fluorescein isothiocyanate (FITC).
纯化方法
egg yolk-derived purified IgY, affinity purified
免疫原
Chickens were immunized with highly purified rabbit IgG isolated from normal pooled rabbit serum.
Optimal working dilutions should be determined for your particular assay conditions.
限制
仅限研究用
状态
Liquid
浓度
1.0 mg/mL
缓冲液
Phosphate buffered saline, 50 % glycerol
储存液
Sodium azide
注意事项
WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
注意事项
Product is photosensitive and should be protected from light. Do not freeze-thaw often