ELISA, WB, ICC, IHC (p), DB
Monoclonal
NI 194-3 (A89-040)
IgG1
HRP
应用备注
Applications: ELISA - Quality tested , FLISA - Quality tested ELISPOT - Reported in literature , FC - Reported in literature , IHC-FS - Reported in literature , IHC-PS - Reported in literature , WB - Reported in literature , Multiplex - Reported in literature , Sep - Reported in literature , Depletion - Reported in literature
Working Dilutions: ELISA AP conjugate 1:1,000 - 1:2,000 HRP conjugate 1:4,000 - 1:16,000 BIOT conjugate 1:5,000 - 1:10,000 FLISA FITC and AF488 conjugates 1:200 - 1:400 PE and AF647 conjugates ≤ 1 g/mL
限制
仅限研究用
浓度
0.5 mg/mL
缓冲液
0.5 mg of purified immunoglobulin in 1.0 mL of borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added
储存液
Without preservative
注意事项
Dilute only prior to immediate use Each reagent is stable for the period shown on the bottle label if stored as directed.
储存条件
4 °C
储存方法
Store at 2-8°C
Pöhler, Emrich, Jordan, Schäfer, Stubenrauch, Staack, Zach, Meir, Faigle: "Comparison of assay formats used for the detection of pre-existing anti-drug antibodies against monoclonal antibodies." in: Bioanalysis, Vol. 14, Issue 13, pp. 923-933, (2022) (PubMed).