山羊 anti-小鼠 IgG (Heavy & Light Chain) Antibody (PE) - Preadsorbed
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- 抗原 See all IgG products
- IgG
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抗原表位
- Heavy & Light Chain
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适用
- 小鼠
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- PE
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应用范围
- Flow Cytometry (FACS), Fluorescence Microscopy (FM)
- 特异性
- IgG (H&L)
- 交叉反应
- 小鼠
- 产品特性
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Anti-Mouse IgG (H&L) Phycoerythrin conjugated antibody generated in goat detects specifically mouse IgG (H&L). This secondary conjugated antibody anti-mouse is ideal for investigators who routinely perform immunomicroscopy and flow cytometry or FACS analysis as well as other antibody-based fluorescent assays requiring extremely low background levels.
Concentration Definition: by absorbance = 82.0 at 565 nm - 纯化方法
- Preadsorption: Solid phase absorption
- 免疫原
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Immunogen: Anti-Mouse IgG was produced by repeated immunization with Mouse IgG whole molecule in goat.
Immunogen Type: Native Protein
- 亚型
- IgG
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- 应用备注
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Application Note: Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 10E6 cells in flow cytometry is approximately 1.0 μg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining. Optimal titers for other applications should be determined by the researcher. As a general guideline dilutions of 1:100 to 1:250 should be suitable for most applications.
Flow Cytometry Dilution: 1:100 - 1:250
IF Microscopy Dilution: 1:100 - 1:250
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 1.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 0.5 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Product is photosensitive and should be protected from light.
- 储存条件
- RT,4 °C
- 有效期
- 12 months
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- 抗原
- IgG
- Abstract
- IgG 产品
- 物质类
- Antibody
- 背景
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Synonyms: goat anti-Mouse IgG phycoerythrin conjugated Antibody, Gt-a-Mouse IgG (H&L) Phycoerythrin conjugated, Mouse IgG(H&L) Phycoerythrin Antibody in goat, goat anti-Mouse IgG PE conjugated Secondary Antibody
Background: Anti-Mouse IgG (H&L) Phycoerythrin conjugated antibody generated in goat detects specifically mouse IgG (H&L). This secondary conjugated antibody anti-mouse is ideal for investigators who routinely perform immunomicroscopy and flow cytometry or FACS analysis as well as other antibody-based fluorescent assays requiring extremely low background levels.
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