山羊 anti-小鼠 IgG, IgM Antibody - Preadsorbed
Rockland 610-101-115
ELISA, WB, IHC
Polyclonal
IgG
unconjugated
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- 抗原 See all IgG, IgM products
- IgG, IgM
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适用
- 小鼠
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宿主
- 山羊
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克隆类型
- 多克隆
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应用范围
- ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
- Supplier Product No.
- 610-101-115
- Supplier
- Rockland
- 原理
- Mouse IgG + IgM (H&L) Antibody Pre-Adsorbed
- 交叉反应 （详细）
- Minimal crossreactivity against to Bv Hs & Hu Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgG and Mouse IgM. No reaction was observed against bovine, horse or human serum proteins.
- 产品特性
- Anti-Mouse IgG3 Antibody generated in goat detects reactivity to Mouse IgG3 (Gamma 3 chain).
- 纯化方法
- This product was prepared from polyspecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
- 过滤
- Sterile filtered
- 免疫原
- Optional[Immunogen]: Mouse IgG and IgM whole molecules
- 亚型
- IgG
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- 应用备注
- Application Note: Anti-Mouse IgG + IgM antibody has been tested by ELISA and is suitable for ELISA, western blot, and immunohistochemistry, as well as other assays requiring lot-to-lot consistency. Immunohistochemistry Dilution: 1:2,000 - 1:10,000 Western Blot Dilution: 1:5,000 - 1:20,000 ELISA Dilution: 1:100,000 Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
, Preservative:0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
- 有效期
- 12 months
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- 抗原
- IgG, IgM
- 别名
- IgG,IgM (IgG, IgM 产品)
- 物质类
- Antibody
- 背景
- Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. Anti-Mouse IgG + IgM Antibody is ideal for investigators in Immunology, Microbiology, and Cell Biology.
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