山羊 anti-小鼠 IgG, IgM Antibody (TRITC) - Preadsorbed
Rockland 610-100-115
FACS, DB, FM, FLISA
Polyclonal
IgG
TRITC
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- 抗原 See all IgG, IgM products
- IgG, IgM
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适用
- 小鼠
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- TRITC
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应用范围
- Flow Cytometry (FACS), Dot Blot (DB), Fluorescence Microscopy (FM), FLISA
- Supplier Product No.
- 610-100-115
- Supplier
- Rockland
- 原理
- Mouse IgG + IgM (H&L) Antibody Rhodamine Conjugated Pre-Adsorbed
- 交叉反应 （详细）
- Minimal crossreactivity against to Bv Hs & Hu Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgG and Mouse IgM. No reaction was observed against bovine, horse or human serum proteins.
- 产品特性
- Anti-Mouse IgG3 Rhodamine Antibody generated in goat detects reactivity to Mouse IgG3 (Gamma 3 chain).
- 纯化方法
- Anti-MOUSE IgG + IgM (H&L) (GOAT) Antibody was prepared from polyspecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
- 免疫原
- Optional[Immunogen]: Mouse IgG and IgM whole molecules
- 亚型
- IgG
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- 应用备注
- Application Note: Anti-MOUSE IgG + IgM (H&L) (GOAT) Rhodamine Antibody has been tested by dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. A working dilution of 1:15,000 to 1:70,000 of the concentration is suggested for this product. Mouse IgG and IgM antibody is suitable for immunoassays including western blot and ELISA. Flow Cytometry Dilution: 1:500 - 1:2,500 FLISA Dilution: 1:10,000 - 1:50,000 IF Microscopy Dilution: 1:1,000 - 1:5,000 Other: FLOW CYTOMETRY 1:500 - 1:2,500
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
, Preservative:0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- IgG, IgM
- 别名
- IgG,IgM (IgG, IgM 产品)
- 物质类
- Antibody
- 背景
- Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. Anti-Mouse IgG + IgM Rhodamine Antibody is ideal for investigators in Immunology, Microbiology, and Cell Biology.
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