山羊 anti-小鼠 IgG, IgM (Heavy & Light Chain) Antibody (TRITC) - Preadsorbed
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- 抗原 See all IgG, IgM products
- IgG, IgM
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抗原表位
- Heavy & Light Chain
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适用
- 小鼠
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- TRITC
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应用范围
- Flow Cytometry (FACS), Fluorescence Microscopy (FM), FLISA
- 特异性
- IgG + IgM (H&L)
- 产品特性
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Anti-MOUSE IgG + IgM (H&L) (GOAT) Antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Mouse IgM antibody is suitable for immunosassays including western blot and ELISA.
Concentration Definition: by UV absorbance at 280 nm - 纯化方法
- Preadsorption: Solid phase absorption
- 免疫原
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Immunogen: Mouse IgG and IgM whole molecules
- 亚型
- IgG
- Labeling Ratio
- 3.3
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- 应用备注
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Application Note: Anti-MOUSE IgG + IgM (H&L) (GOAT) Antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. A working dilution of 1:15,000 to 1:70,000 of the concentration is suggested for this product. Mouse IgG and IgM antibody is suitable for immunoassays including western blot and ELISA.
FLISA Dilution: 1:10,000 - 1:50,000
Flow Cytometry Dilution: 1:500 - 1:2,500
IF Microscopy Dilution: 1:1,000 - 1:5,000
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 1.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Product is photosensitive and should be protected from light.
- 储存条件
- RT,4 °C,-20 °C
- 有效期
- 12 months
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- 抗原
- IgG, IgM
- 别名
- IgG + IgM (IgG, IgM 产品)
- 物质类
- Antibody
- 背景
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Synonyms: Goat Anti-Mouse IgG + IgM Antibody Fluorescein Conjugated Pre-Adsorbed, Goat Anti-Mouse IgG/IgM Antibody Fluorescein Conjugated
Background: Anti-Mouse IgG + IgM Rhodamine Antibody generated in Goat detects reactivity to Mouse IgG and IgM. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. Anti-Mouse IgG + IgM Rhodamine Antibody is ideal for investigators in Immunology, Microbiology, and Cell Biology.
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