ELISA: 1:10,000 - 1:100,000. Western Blot: 1:1000 - 1:10,000. Immunohistochemistry on frozen sections: 1:200 - 1:500.
Other applications not tested. Otimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
浓度
1.0 mg/mL
缓冲液
PBS containing 0.09 % ProclinTM300 and 0.2 % Bovine Serum Albumin
储存液
ProClin
注意事项
This product contains ProClinTM: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
注意事项
Avoid repeated freezing and thawing. Dilute only prior to immediate use Centrifuge product if not completely clear after standing at room temperature. Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
储存条件
4 °C/-20 °C
储存方法
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -28 °C for longer.