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山羊 anti-人 Ig (Chain kappa), (Light Chain) Antibody (Biotin)

ELISA, IHC, WB, IEP, IM Polyclonal Biotin F(ab')2 fragment
产品编号 ABIN117713
发货至: 中国
  • 抗原 See all Ig products
    Ig
    片段
    F(ab')2 fragment
    抗原表位
    • 88
    • 86
    • 68
    • 32
    • 4
    • 4
    • 2
    • 1
    • 1
    • 1
    Chain kappa, Light Chain
    适用
    • 108
    • 61
    • 26
    • 17
    • 9
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    宿主
    • 98
    • 73
    • 36
    • 20
    • 2
    • 1
    • 1
    • 1
    山羊
    克隆类型
    • 111
    • 95
    多克隆
    标记
    • 59
    • 29
    • 22
    • 21
    • 17
    • 16
    • 10
    • 10
    • 5
    • 4
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Biotin
    应用范围
    • 162
    • 125
    • 86
    • 67
    • 41
    • 26
    • 25
    • 14
    • 12
    • 9
    • 8
    • 6
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Immunoelectrophoresis (IEP), Immunomicroscopy (IM)
    产品特性
    Molar Ratio: 10-20 BAC molecules per Goat IgG Fab molecule.
    纯化方法
    Immunoaffinity chromatography.
    免疫原
    Human kappa (kappa) light chain.
  • 应用备注
    Suitable for use in Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy aswell as other antibody based assays using streptavidin or avidin conjugates requiringextremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency,high titer and specificity. Recommended Dilutions: This product has been assayed against 1.0 μg of Human IgG in astandard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS(2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes atroom temperature. A working dilution of 1: 3,000 to 1: 8,500 of the reconstitutionconcentration is suggested for this product.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    限制
    仅限研究用
  • 溶解方式
    Restore with 1.0 mL of deionized water (or equivalent).
    浓度
    1.0 mg/mL (by UV absorbance at 280 nm)
    缓冲液
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, with 10 mg/mL Bovine Serum Albumin (BSA) IgG and Protease free as stabilizer and 0.01 % (w/v) Sodium Azide as preservative.
    储存液
    Sodium azide
    注意事项
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Avoid repeated freezing and thawing. Dilute only prior to immediate use
    储存条件
    4 °C/-20 °C
    储存方法
    Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.
  • 抗原
    Ig
    别名
    Immunoglobulin (Ig 产品)
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