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山羊 anti-小鼠 IgG1 Antibody (Atto 647N)

Name: 山羊 anti-小鼠 IgG1 Antibody (Atto 647N)
SKU: ABIN1118519
Availability: OutOfStock

Rockland 610-156-040 WB, FACS, FLISA, DB, FM Polyclonal IgG Atto 647N

Western Blot of ATTO 647N conjugated Goat anti-Mouse IgG1 (gamma 1 chain) Pre-adsorbed secondary antibody. (山羊 anti-小鼠 IgG1 Antibody (Atto 647N))

1 image

(2 references)

产品编号 ABIN1118519

Supplier Product No.: 610-156-040

发货至: 中国

Datasheet as PDF

抗原 See all IgG1 products

IgG1
适用
94

24

23

9

7

5

4

4

3

2

1
小鼠
宿主
60

42

39

15

10

6

1
山羊
克隆类型
92

60
多克隆
标记
46

21

21

18

11

8

7

4

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Atto 647N
应用范围
90

59

50

46

29

19

18

16

13

11

10

9

8

6

4

3

2

2

2
Western Blotting (WB), Flow Cytometry (FACS), FLISA, Dot Blot (DB), Fluorescence Microscopy (FM)

Supplier Product No.

610-156-040

Supplier

Rockland

原理

Mouse IgG1 (Gamma 1 chain) ATTO 647N Conjugated Antibody pre-absorbed

交叉反应（详细）

Minimal crossreactivity against Bv, Hu, and Rb Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse Serum, Mouse IgG and Mouse IgG1. No reaction was observed against Bovine, Human, and Rabbit Serum Proteins. Specificity was confirmed by ELISA at less than 1 % of target signal.

产品特性

Anti-Mouse IgG ATTO 594 Antibody generated in goat detects reactivity to Mouse IgG.

纯化方法

Anti-Mouse IgG1 antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG1 coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

免疫原

Immunogen: Mouse IgG1 heavy chain
Immunogen Type: Native Protein

亚型

IgG
Goat anti-Mouse IgG1 (Heavy Chain) antibody (HRP)
ELISA, WB, IHC Polyclonal IgG HRP

Goat anti-Mouse IgG1 (Heavy Chain) antibody (Alkaline Phosphatase (AP))
ELISA, WB, IHC Polyclonal IgG Alkaline Phosphatase (AP)

Rat anti-Mouse IgG1 antibody
ELISA, FACS Monoclonal SB77e IgG2b unconjugated

Goat anti-Mouse IgG1 (Heavy Chain) antibody
ELISA, WB, IHC, FACS Polyclonal IgG unconjugated

Goat anti-Mouse IgG1 (Heavy Chain) antibody (FITC)
ELISA, IHC, FACS Polyclonal IgG FITC

Goat anti-Mouse IgG1 (Heavy Chain) antibody (TRITC)
ELISA, IHC, FACS Polyclonal IgG TRITC

Goat anti-Mouse IgG1 (Heavy Chain) antibody (Biotin)
ELISA, WB, IHC, FACS Polyclonal IgG Biotin

Goat anti-Mouse IgG1 (Heavy Chain) antibody (Cy5)
IHC, FACS Polyclonal IgG Cy5

Goat anti-Mouse IgG1 antibody (PE) - Preadsorbed
ELISA, FACS Polyclonal IgG PE F(ab')2 fragment

Goat anti-Mouse IgG1 antibody (PE) - Preadsorbed
ELISA, FACS Polyclonal IgG PE

应用备注

Application Note: Anti-Mouse IgG1 ATTO 647N Antibody has been tested by dot blot and western blot and is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this ATTO conjugate matches the principle output wavelengths of most common fluorescence instrumentation. Flow Cytometry Dilution: 1:500 - 1:2,500 Western Blot Dilution: >1:10,000 FLISA Dilution: >1:20,000 IF Microscopy Dilution: >1:5,000 Other: User Optimized

限制

仅限研究用
状态

Lyophilized

溶解方式

Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 500 µL

浓度

1.0 mg/mL

缓冲液

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
, Preservative:0.01 % (w/v) Sodium Azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store vial at 4° C prior to restoration. For extended storage aliquot secondary antibody and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

有效期

12 months
Schilling, Ali, Leonhardt, Borst, Pujol-Martí: "Transcriptional control of morphological properties of direction-selective T4/T5 neurons in Drosophila." in: Development (Cambridge, England), Vol. 146, Issue 2, (2019) (PubMed).

Hruska, Henderson, Le Marchand, Jafri, Dalva: "Synaptic nanomodules underlie the organization and plasticity of spine synapses." in: Nature neuroscience, Vol. 21, Issue 5, pp. 671-682, (2019) (PubMed).
抗原

IgG1

Abstract

IgG1 产品

物质类

Antibody

背景

Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. IgG1 chain constitutes 66 % of the IgG subclass and has a high affinity for binding to the Fc receptor of phagocytic cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.