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山羊 anti-小鼠 IgG1 (Heavy Chain) Antibody (Atto 647N) - Preadsorbed

Name: 山羊 anti-小鼠 IgG1 (Heavy Chain) Antibody (Atto 647N) - Preadsorbed
Brand: antibodies-online
SKU: ABIN1118519
Availability: OutOfStock

WB, FACS, FLISA, FM Polyclonal IgG Atto 647N

Western Blot of ATTO 647N conjugated Goat anti-Mouse IgG1 (gamma 1 chain) Pre-adsorbed secondary antibody. (山羊 anti-小鼠 IgG1 (Heavy Chain) Antibody (Atto 647N) - Preadsorbed)

1 image

(2 references)

产品编号 ABIN1118519

发货至: 中国

Datasheet as PDF

抗原 See all IgG1 products

IgG1
抗原表位
51

26

17

6

5

3

3

2

2

1

1

1

1

1
Heavy Chain
适用
95

24

23

7

7

5

4

4

3

2

1
小鼠
宿主
62

42

37

14

10

6

1
山羊
克隆类型
94

57
多克隆
标记
46

20

19

19

11

8

7

5

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Atto 647N
应用范围
85

58

52

46

31

20

18

15

11

10

10

9

5

4

3

2

2

2
Western Blotting (WB), Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)

特异性

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse Serum and Mouse IgG.
Specificity was confirmed by ELISA at less than 0.5 % of target signal.

交叉反应

小鼠

产品特性

ATTO Dye Conjugated Secondary Antibodies are designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. When choosing a secondary antibody, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

纯化方法

Preadsorption: Solid phase absorption

免疫原

Immunogen: Mouse IgG1 heavy chain
Immunogen Type: Native Protein

亚型

IgG

Labeling Ratio

1.3
Goat anti-Mouse IgG1 (Heavy Chain) antibody (HRP)
ELISA, WB, IHC Polyclonal IgG HRP

Goat anti-Mouse IgG1 (Heavy Chain) antibody (Alkaline Phosphatase (AP))
ELISA, WB, IHC Polyclonal IgG Alkaline Phosphatase (AP)

Rabbit anti-Mouse IgG1 (Heavy Chain) antibody - Preadsorbed
ELISA, WB, IHC Polyclonal IgG unconjugated

Rat anti-Mouse IgG1 antibody
ELISA, FACS Monoclonal SB77e IgG2b unconjugated

Goat anti-Mouse IgG1 (Heavy Chain) antibody
ELISA, WB, IHC, FACS Polyclonal IgG unconjugated

Rabbit anti-Mouse IgG1 (Heavy Chain) antibody (Texas Red (TR)) - Preadsorbed
FACS, FLISA, FM Polyclonal IgG Texas Red (TR)

Goat anti-Mouse IgG1 (Heavy Chain) antibody (FITC)
ELISA, IHC, FACS Polyclonal IgG FITC

Goat anti-Mouse IgG1 (Heavy Chain) antibody (TRITC)
ELISA, IHC, FACS Polyclonal IgG TRITC

Goat anti-Mouse IgG1 (Heavy Chain) antibody (Biotin)
ELISA, WB, IHC, FACS Polyclonal IgG Biotin

Rabbit anti-Mouse IgG1 (Heavy Chain) antibody (Alkaline Phosphatase (AP))
ELISA, WB, IHC Polyclonal IgG Alkaline Phosphatase (AP)

应用备注

Application Note: ATTO Dye Conjugated Secondary Antibodies are designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this ATTO conjugate matches the principle output wavelengths of most common fluorescence instrumentation.
FLISA Dilution: >1:20,000
Flow Cytometry Dilution: 1:500 - 1:2,500
Western Blot Dilution: >1:10,000
IF Microscopy Dilution: >1:5,000

说明

The emission spectra for this ATTO conjugate matches the principle output wavelengths of most common fluorescence instrumentation.

限制

仅限研究用
状态

Lyophilized

溶解方式

Reconstitution Volume: 500 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)

浓度

1.0 mg/mL

缓冲液

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Avoid cycles of freezing and thawing.
Product is photosensitive and should be protected from light.

储存条件

RT,4 °C,-20 °C

储存方法

Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -24 °C or below. This product is stable for several weeks at 4 °C as an undiluted liquid.
Schilling, Ali, Leonhardt, Borst, Pujol-Martí: "Transcriptional control of morphological properties of direction-selective T4/T5 neurons in Drosophila." in: Development (Cambridge, England), Vol. 146, Issue 2, (2019) (PubMed).

Hruska, Henderson, Le Marchand, Jafri, Dalva: "Synaptic nanomodules underlie the organization and plasticity of spine synapses." in: Nature neuroscience, Vol. 21, Issue 5, pp. 671-682, (2019) (PubMed).
抗原

IgG1

Abstract

IgG1 产品

物质类

Antibody

背景

Synonyms: Goat anti-mouse IgG1 antibody ATTO647N conjugation, goat anti-mouse IgG1 (gamma 1) ATTO 647N conjugated antibody
Background: Anti-Mouse IgG1 ATTO 647N Antibody generated in goat detects reactivity to Mouse IgG1 (Gamma 1 chain). Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. IgG1 chain constitutes 66 % of the IgG subclass and has a high affinity for binding to the Fc receptor of phagocytic cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.