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山羊 anti-小鼠 IgG3 Antibody (HRP)

Name: 山羊 anti-小鼠 IgG3 Antibody (HRP)
SKU: ABIN1118505
Availability: OutOfStock

Rockland 610-103-043 ELISA, WB, IHC Polyclonal IgG HRP

(2 references)

产品编号 ABIN1118505

Supplier Product No.: 610-103-043

发货至: 中国

Datasheet as PDF

抗原 See all IgG3 products

IgG3
适用
75

19

3
小鼠
宿主
35

34

17

10

1
山羊
克隆类型
62

26
多克隆
标记
19

13

12

10

7

5

5

4

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
HRP
应用范围
56

41

38

30

27

19

12

11

10

6

6

4

4

3

3

2

2

2

1
ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Supplier Product No.

610-103-043

Supplier

Rockland

原理

Mouse IgG3 Antibody Peroxidase Conjugated Pre-adsorbed

交叉反应（详细）

Minimal crossreactivity against Bv, Hu, and Rb Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase, anti-Goat Serum, Mouse Serum and Mouse IgG3. No reaction was observed against Bovine, Human, and Rabbit Serum Proteins. Specificity was confirmed by ELISA at less than 1 % of target signal.

产品特性

Anti-Mouse IgG2b Peroxidase Antibody generated in goat detects reactivity to Mouse IgG2b (Gamma 2b chain).

免疫原

Immunogen: Mouse IgG3 heavy chain
Immunogen Type: Native Protein

亚型

IgG
Goat anti-Mouse IgG3 antibody (HRP)
ELISA, FLISA Polyclonal IgG HRP

Goat anti-Mouse IgG3 (Heavy Chain) antibody (HRP)
ELISA, WB, IHC Polyclonal IgG HRP

Goat anti-Mouse IgG3 antibody
ELISA, FLISA Polyclonal IgG unconjugated

Goat anti-Mouse IgG3 (Heavy Chain) antibody (Alkaline Phosphatase (AP))
ELISA, WB, IHC Polyclonal IgG Alkaline Phosphatase (AP)

Goat anti-Mouse IgG3 antibody (FITC)
ELISA, FLISA Polyclonal IgG FITC

Rat anti-Mouse IgG3 antibody
ELISA, FACS, FLISA Monoclonal SB76b IgG2a unconjugated

Goat anti-Mouse IgG3 (Heavy Chain) antibody
ELISA, WB, IHC, FACS Polyclonal IgG unconjugated

Rat anti-Mouse IgG3 antibody (Biotin)
ELISA, FACS, FLISA Monoclonal SB76b IgG2a Biotin

Goat anti-Mouse IgG3 antibody (Biotin) - Preadsorbed
ELISA, FACS Polyclonal IgG Biotin F(ab')2 fragment

Rat anti-Mouse IgG3 antibody (HRP)
ELISA, FACS, FLISA Monoclonal SB76b IgG2a HRP

应用备注

Application Note: Anti-Mouse IgG3 peroxidase antibody has been tested by ELISA and is ideal for western blotting, Immunohistochemistry and ELISA as well as other antibody detection methods and anti IgG2b antibody based assays. Mouse IgG3 secondary antibody is available in a variety of formats. HRP Conjugated Secondary Antibodies are ideal for western blot and are available in a variety of formats and conjugate types. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:2,000 - 1:10,000 ELISA Dilution: 1:20,000 - 1:100,000 Other: User Optimized

限制

仅限研究用
状态

Lyophilized

溶解方式

Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL

浓度

1.0 mg/mL

缓冲液

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
, Preservative:0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

储存液

Gentamicin sulfate

注意事项

This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store vial at 4° C prior to restoration. For extended storage aliquot antibody and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Anti-Mouse IgG HRP secondary antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

有效期

12 months
Guo, Kong, Peng, Long, Zhou, Shu: "Transcription factor NF‑YA promotes a malignant phenotype by upregulating fatty acid synthase expression." in: Molecular medicine reports, Vol. 14, Issue 6, pp. 5007-5014, (2017) (PubMed).

Chen, Ruan, Long, Peng, Zhou, Liu, Zhou, Liu et al.: "Blocking fatty acid synthase inhibits tumor progression of human osteosarcoma by regulating the human epidermal growth factor receptor 2/phosphoinositide 3-kinase/protein kinase B signaling pathway ..." in: Experimental and therapeutic medicine, Vol. 13, Issue 5, pp. 2411-2416, (2017) (PubMed).
抗原

IgG3

Abstract

IgG3 产品

物质类

Antibody

背景

Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. IgG3 comprises almost 10 % of the IgG subclass and has a high affinity for binding to the Fc receptor of phagocytic cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.