ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
特异性
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse Serum and Mouse IgG3. Specificity was confirmed by ELISA at less than 0.5 % of target signal.
产品特性
HRP Conjugated Secondary Antibodies are ideal for western blot and are available in a variety of formats and conjugate types. When choosing a secondary antibody, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
纯化方法
Preadsorption: Solid phase absorption
免疫原
Mouse IgG3 heavy chain Immunogen Type: Native Protein
Anti-Mouse IgG3 peroxidase Secondary antibody reagents are ideal for western blotting, Immunohistochemistry and ELISA as well as other antibody detection methods and anti IgG2b antibody based assays.
限制
仅限研究用
状态
Lyophilized
溶解方式
Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL
浓度
1.0 mg/mL
缓冲液
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
储存液
Gentamicin sulfate
注意事项
Avoid repeated freezing and thawing. Dilute only prior to immediate use Centrifuge product if not completely clear after standing at room temperature. Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
储存条件
4 °C/-20 °C
储存方法
Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -24 °C or below. This product is stable for several weeks at 4 °C as an undiluted liquid.
有效期
12 months
Guo, Kong, Peng, Long, Zhou, Shu: "Transcription factor NF‑YA promotes a malignant phenotype by upregulating fatty acid synthase expression." in: Molecular medicine reports, Vol. 14, Issue 6, pp. 5007-5014, (2017) (PubMed).
Chen, Ruan, Long, Peng, Zhou, Liu, Zhou, Liu et al.: "Blocking fatty acid synthase inhibits tumor progression of human osteosarcoma by regulating the human epidermal growth factor receptor 2/phosphoinositide 3-kinase/protein kinase B signaling pathway ..." in: Experimental and therapeutic medicine, Vol. 13, Issue 5, pp. 2411-2416, (2017) (PubMed).