山羊 anti-小鼠 IgG2b Antibody (Texas Red (TR))
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- 抗原 See all IgG2b products
- IgG2b
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适用
- 小鼠
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- Texas Red (TR)
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应用范围
- Immunohistochemistry (IHC), Western Blotting (WB), FLISA, Dot Blot (DB), Fluorescence Microscopy (FM)
- Supplier Product No.
- 610-109-042
- Supplier
- Rockland
- 原理
- Mouse IgG2b Antibody Texas Red™ Conjugated Pre-adsorbed
- 交叉反应 （详细）
- Minimal crossreactivity against Bv, Hu, and Rb Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse Serum and Mouse IgG2b. No reaction was observed against Bovine, Human, and Rabbit Serum Proteins. Specificity was confirmed by ELISA at less than 1 % of target signal.
- 产品特性
- Anti-Mouse IgG2a Texas Red Antibody generated in goat detects reactivity to Mouse IgG2a (Gamma 2a chain).
- 纯化方法
- Anti-MOUSE IgG2b (Gamma 2b chain) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
- 免疫原
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Immunogen: Mouse IgG2b heavy chain
Immunogen Type: Native Protein
- 亚型
- IgG
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Goat anti-Mouse IgG2b (Heavy Chain) antibody (HRP)
ELISA, IHC, WB Polyclonal IgG HRP
Goat anti-Mouse IgG2b (Heavy Chain) antibody (FITC)ELISA, IHC, FACS Polyclonal IgG FITC
Goat anti-Mouse IgG2b (Heavy Chain) antibody (TRITC)ELISA, IHC, FACS Polyclonal IgG TRITC
Goat anti-Mouse IgG2b antibody (Alkaline Phosphatase (AP))ELISA, FLISA Polyclonal IgG Alkaline Phosphatase (AP)
Goat anti-Mouse IgG2b antibodyELISA, FLISA Polyclonal IgG unconjugated
Goat anti-Mouse IgG2b (Heavy Chain) antibody (Alkaline Phosphatase (AP))ELISA, IHC, WB Polyclonal IgG Alkaline Phosphatase (AP)
Rat anti-Mouse IgG2b antibodyELISA, FACS, FLISA Monoclonal SB74g IgG2b unconjugated
Goat anti-Mouse IgG2b antibody (Biotin)ELISA, FLISA Polyclonal IgG Biotin
Rat anti-Mouse IgG2b antibody (FITC)ELISA, FACS, FLISA Monoclonal SB74g IgG2b FITC
Rat anti-Mouse IgG2b antibody (Biotin)ELISA, FACS, FLISA Monoclonal SB74g IgG2b Biotin
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- 应用备注
- Application Note: Anti-Mouse IgG2b Texas Red™ Conjugated antibody has been tested by dot blot and is suitable for ELISA, Immunohistochemistry, western blotting, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting as well as other anti IgG2a antibody based assays. Mouse IgG2b secondary antibody is available in a variety of formats. This product is suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:4,000 - 1:20,000 FLISA Dilution: 1:10,000 - 1:50,000 IF Microscopy Dilution: 1:1,000 - 1:5,000 Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
, Preservative:0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- IgG2b
- Abstract
- IgG2b 产品
- 物质类
- Antibody
- 背景
- Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. IgG2, the second largest of IgG isotypes, comprises almost 25 % of IgG and has a low affinity for binding to the Fc receptor of phagocytic cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
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