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山羊 anti-小鼠 IgG2a Antibody (FITC)

Name: 山羊 anti-小鼠 IgG2a Antibody (FITC)
SKU: ABIN1118478
Availability: OutOfStock

Rockland 610-102-041 FACS, IHC, WB, FLISA, DB, FM Polyclonal IgG FITC

Dot Blot of Goat anti-Mouse IgG2a Antibody Fluorescein Conjugated Pre-absorbed. (山羊 anti-小鼠 IgG2a Antibody (FITC))

1 image

(3 references)

产品编号 ABIN1118478

Supplier Product No.: 610-102-041

发货至: 中国

Datasheet as PDF

抗原 See all IgG2a products

IgG2a
适用
98

21

1
小鼠
宿主
56

28

16

15

5
山羊
克隆类型
70

31
多克隆
标记
23

14

12

12

9

5

5

5

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
FITC
应用范围
63

46

40

34

23

18

16

10

10

8

4

4

3

3

2

2

2

1

1
Flow Cytometry (FACS), Immunohistochemistry (IHC), Western Blotting (WB), FLISA, Dot Blot (DB), Fluorescence Microscopy (FM)

Supplier Product No.

610-102-041

Supplier

Rockland

原理

Mouse IgG2a Antibody Fluorescein Conjugated Pre-adsorbed

交叉反应（详细）

Minimal crossreactivity against Bv, Hu, and Rb Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-Goat Serum, Mouse Serum and Mouse IgG2a. Specificity was confirmed by ELISA at less than 1 % cross-reactivity against other mouse heavy or light chain isotypes. No reaction was observed against Bovine, Human, and Rabbit Serum Proteins. Specificity was confirmed by ELISA at less than 1 % of target signal.

产品特性

Anti-Mouse IgG1 Fluorescein Antibody generated in goat detects reactivity to Mouse IgG1 (Gamma 1 chain).

纯化方法

MOUSE IgG2a Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

免疫原

Immunogen: Mouse IgG2a heavy chain
Immunogen Type: Native Protein

亚型

IgG
Goat anti-Mouse IgG2a (Heavy Chain) antibody (HRP)
ELISA, IHC, WB Polyclonal IgG HRP

Rat anti-Mouse IgG2a antibody
ELISA, FACS Monoclonal SB84a IgG1 unconjugated

Rat anti-Mouse IgG2a antibody (HRP)
ELISA, IHC Monoclonal SB84a IgG1 HRP

Rat anti-Mouse IgG2a antibody (PE)
ELISA, FACS Monoclonal SB84a IgG1 PE

Goat anti-Mouse IgG2a (Heavy Chain) antibody (TRITC)
ELISA, FACS, IHC Polyclonal IgG TRITC

Goat anti-Mouse IgG2a antibody (PE) - Preadsorbed
ELISA, FACS Polyclonal IgG PE

Goat anti-Mouse IgG2a antibody (APC) - Preadsorbed
ELISA, FACS Polyclonal IgG APC

Goat anti-Mouse IgG2a antibody (Alkaline Phosphatase (AP))
ELISA, FLISA Polyclonal IgG Alkaline Phosphatase (AP)

Goat anti-Mouse IgG2a antibody
ELISA, FLISA Polyclonal IgG unconjugated

Goat anti-Mouse IgG2a (Heavy Chain) antibody (Alkaline Phosphatase (AP))
ELISA, IHC, WB Polyclonal IgG Alkaline Phosphatase (AP)

应用备注

Application Note: Mouse IgG2a Fluorescein antibody has been tested by dot blot and is available in a variety of formats. FITC Conjugated Secondary Antibodies are designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Flow Cytometry Dilution: 1:200 - 1:500 Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:2,000 - 1:10,000 FLISA Dilution: 1:10,000 - 1:50,000 IF Microscopy Dilution: 1:1,000 - 1:5,000 Other: User Optimized

限制

仅限研究用
状态

Lyophilized

溶解方式

Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL

浓度

1.7 mg/mL

缓冲液

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
, Preservative:0.01 % (w/v) Sodium Azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store anti mouse secondary antibody at 4° C prior to restoration. For extended storage aliquot antibody and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

有效期

12 months
Musante, Li, Kanyo, Lam, Colangelo, Cheng, Brody, Greengard, Le Novère, Nairn: "Reciprocal regulation of ARPP-16 by PKA and MAST3 kinases provides a cAMP-regulated switch in protein phosphatase 2A inhibition." in: eLife, Vol. 6, (2018) (PubMed).

Carlyle, Kitchen, Kanyo, Voss, Pletikos, Sousa, Lam, Gerstein, Sestan, Nairn: "A multiregional proteomic survey of the postnatal human brain." in: Nature neuroscience, Vol. 20, Issue 12, pp. 1787-1795, (2017) (PubMed).

Andrade, Musante, Horiuchi, Matsuzaki, Brody, Wu, Greengard, Taylor, Nairn: "ARPP-16 Is a Striatal-Enriched Inhibitor of Protein Phosphatase 2A Regulated by Microtubule-Associated Serine/Threonine Kinase 3 (Mast 3 Kinase)." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 37, Issue 10, pp. 2709-2722, (2017) (PubMed).
抗原

IgG2a

Abstract

IgG2a 产品

物质类

Antibody

背景

Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. IgG2, the second largest of IgG isotypes, comprises almost 25 % of IgG and has a low affinity for binding to the Fc receptor of phagocytic cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.