山羊 anti-小鼠 IgG1 (Heavy Chain) Antibody (FITC) - Preadsorbed
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- 抗原 See all IgG1 products
- IgG1
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抗原表位
- Heavy Chain
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适用
- 小鼠
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- FITC
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)
- 特异性
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Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Mouse IgG and Mouse Serum.
Specificity was confirmed by ELISA at less than 0.5 % of target signal. - 交叉反应
- 小鼠
- 产品特性
- FITC Conjugated Secondary Antibodies are designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
- 纯化方法
- Preadsorption: Solid phase absorption
- 免疫原
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Immunogen: Mouse IgG heavy chain
Immunogen Type: Native Protein
- 亚型
- IgG
- Labeling Ratio
- 2.8
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Goat anti-Mouse IgG1 (Heavy Chain) antibody (HRP)
ELISA, WB, IHC Polyclonal IgG HRP
Goat anti-Mouse IgG1 (Heavy Chain) antibody (Alkaline Phosphatase (AP))ELISA, WB, IHC Polyclonal IgG Alkaline Phosphatase (AP)
Rabbit anti-Mouse IgG1 (Heavy Chain) antibody - PreadsorbedELISA, WB, IHC Polyclonal IgG unconjugated
Rat anti-Mouse IgG1 antibodyELISA, FACS Monoclonal SB77e IgG2b unconjugated
Goat anti-Mouse IgG1 (Heavy Chain) antibodyELISA, WB, IHC, FACS Polyclonal IgG unconjugated
Rabbit anti-Mouse IgG1 (Heavy Chain) antibody (Texas Red (TR)) - PreadsorbedFACS, FLISA, FM Polyclonal IgG Texas Red (TR)
Goat anti-Mouse IgG1 (Heavy Chain) antibody (FITC)ELISA, IHC, FACS Polyclonal IgG FITC
Goat anti-Mouse IgG1 (Heavy Chain) antibody (TRITC)ELISA, IHC, FACS Polyclonal IgG TRITC
Goat anti-Mouse IgG1 (Heavy Chain) antibody (Biotin)ELISA, WB, IHC, FACS Polyclonal IgG Biotin
Rabbit anti-Mouse IgG1 (Heavy Chain) antibody (Alkaline Phosphatase (AP))ELISA, WB, IHC Polyclonal IgG Alkaline Phosphatase (AP)
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- 应用备注
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Immunohistochemistry Dilution: 1:1,000 - 1:5,000
Application Note: Mouse IgG1 FITC conjugated secondary antibody reagents are ideal for Flow Cytometry, Immunohistochemistry and Immunofluorescence Microscopy as well as other antibody detection methods. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
FLISA Dilution: 1:10,000 - 1:50,000
Flow Cytometry Dilution: 1:200 - 1:500
Western Blot Dilution: 1:2,000 - 1:10,000
IF Microscopy Dilution: 1:1,000 - 1:5,000
- 说明
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Excitation/Emission wavelength: 494 nm/514 nm
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 1.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Product is photosensitive and should be protected from light.
- 储存条件
- RT,4 °C,-20 °C
- 储存方法
- Store vial at 4 °C before opening. This product is stable at 4 °C as an undiluted liquid.
- 有效期
- 12 months
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- 抗原
- IgG1
- Abstract
- IgG1 产品
- 物质类
- Antibody
- 背景
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Synonyms: Goat anti-mouse IgG1 antibody fluorescein conjugation, goat anti-mouse IgG1 (gamma 1) FITC conjugated antibody
Background: Anti-Mouse IgG1 Fluorescein Antibody generated in goat detects reactivity to Mouse IgG1 (Gamma 1 chain). Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. IgG1 chain constitutes 66 % of the IgG subclass and has a high affinity for binding to the Fc receptor of phagocytic cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
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