兔 anti-绵羊 IgG (Heavy & Light Chain) Antibody (TRITC)
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- 抗原 See all IgG products
- IgG
- 片段
- F(ab')2 fragment
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抗原表位
- Heavy & Light Chain
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适用
- 绵羊
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- TRITC
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应用范围
- Fluorescence Microscopy (FM), Dot Blot (DB)
- Supplier Product No.
- 713-400-002
- Supplier
- Rockland
- 原理
- F(ab')2 Sheep IgG (H&L) Antibody Rhodamine Conjugated
- 交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Sheep IgG and Sheep Serum. No reaction was observed against anti-Pepsin or anti-Rabbit IgG F(c).
- 产品特性
- F(ab')2 Anti-Rat IgG (H&L) Antibody generated in donkey detects rat IgG. Representing approximately 75 % of serum immunoglobulins, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunofluorescence, IHC, and other immunoassays. This F(ab')2 Anti-Rat IgG Antibody is conjugated to Texas Red™.
- 纯化方法
- This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
- 免疫原
- Optional[Immunogen]: Sheep IgG whole molecule
- 亚型
- IgG
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Donkey anti-Sheep IgG antibody (DyLight 680) - Preadsorbed
WB, FLISA, FM Polyclonal IgG DyLight 680
Rabbit anti-Sheep IgG (Heavy & Light Chain) antibody (Biotin) - PreadsorbedELISA, IHC, WB, DB Polyclonal IgG Biotin
Donkey anti-Sheep IgG (Heavy & Light Chain) antibody (FITC)FACS, FLISA, FM, DB Polyclonal IgG FITC
Rabbit anti-Sheep IgG (Heavy & Light Chain) antibody (TRITC)FACS, FLISA, FM, DB Polyclonal IgG TRITC
Rabbit anti-Sheep IgG (Heavy & Light Chain) antibody (FITC)WB, FACS, FLISA, FM, DB Polyclonal IgG FITC
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- 应用备注
- Application Note: F(ab')2 Anti-Sheep IgG Rhodamine Antibody has been tested by dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. IF Microscopy Dilution: 1:500-1:2,500
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 500 µL
- 浓度
- 1.0 mg/mL
- 缓冲液
- Optional[Buffer]: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- IgG
- Abstract
- IgG 产品
- 物质类
- Antibody
- 背景
- F(ab')2 Anti-Sheep IgG (H&L) Antibody generated in rabbit detects sheep IgG. Representing approximately 75 % of serum immunoglobulins, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunofluorescence, IHC, and other immunoassays. This F(ab')2 Anti-Sheep IgG Antibody is conjugated to rhodamine.
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