兔 anti-犬 IgG (Heavy & Light Chain) Antibody (Biotin) - Preadsorbed
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- 抗原 See all IgG products
- IgG
- 片段
- F(ab')2 fragment
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抗原表位
- Heavy & Light Chain
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适用
- 犬
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- Biotin
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应用范围
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- 特异性
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum, Dog IgG and Dog Serum.
- 纯化方法
- Preadsorption: Solid phase absorption
- 免疫原
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Immunogen: Dog IgG whole molecule
- 亚型
- IgG
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Rabbit anti-Dog IgG (Fc Region) antibody - Preadsorbed
ELISA, IHC, WB Polyclonal IgG unconjugated
Rabbit anti-Dog IgG (Fc Region) antibody (Alkaline Phosphatase (AP)) - PreadsorbedELISA, IHC, WB Polyclonal IgG Alkaline Phosphatase (AP)
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- 应用备注
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Immunohistochemistry Dilution: 1:1,000-1:5,000
Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
ELISA Dilution: 1:20,000-1:100,000
Western Blot Dilution: 1:2,000-1:10,000
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 500 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 1 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
- 注意事项
- Aliquot to Avoid repeated freezing and thawing.
- 储存条件
- RT,4 °C,-20 °C
- 储存方法
- Store vial at 4 °C prior to opening. This product is stable for several weeks at 4 °C as an undiluted liquid. For extended storage aliquot contents and freeze at -24 °C or below.
- 有效期
- 12 months
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- 抗原
- IgG
- Abstract
- IgG 产品
- 物质类
- Antibody
- 背景
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Synonyms: Rabbit F(ab')2 Anti-Dog Biotin Conjugated Antibody, Rabbit Fab2 Anti-Dog Antibody Biotin Conjugation, Rabbit Fab'2 Anti-Dog Antibody Biotin Conjugation
Background: F(ab')2 Antibody was generated by enzymatic cleavage and subsequent separation from the Fc fragment. Because of their smaller size, F(ab)2 fragments offer several advantages over intact antibodies for use in certain immunochemical techniques and experimental applications. F(ab)2 fragments penetrate tissue samples and show better antigen recognition and signal generation in IHC. F(ab)2 fragments lack the Fc region and therefore do not bind Fc receptors which effectively lowers background staining. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.
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